Nejvíce citovaný článek - PubMed ID 17176770
Establishment and follow-up of bifidobacterial species in the gut of healthy bottle-fed infants of 1-4 months age
Twenty-eight exclusively breast-fed healthy infants and 16 infants also exclusively breast-fed with allergic colitis (aged 85 +/- 60 and 98 +/- 58 d, respectively) were screened for differences in fecal flora. Bifidobacteria were detected in 23 healthy infants and only in 4 fecal samples of infants with allergic colitis. All bifidobacteria-free infants possessed Gram-positive regular rods as a major group of their fecal flora. These bacteria were identified as clostridia using genus-specific FISH probe. Infants with allergy colitis possessed significantly lower counts of bifidobacteria and total anaerobes and significantly higher counts of clostridia in their feces. In healthy infants, Bifidobacterium longum was the most frequently found species (54.5% of the samples), followed by B. adolescentis (20.0), B. breve (18.2), B. bifidum (16.4), B. dentium (10.9) and B. pseudocatenulatum (1.80). Bifidobacterial isolates from two babies with allergic colitis were identified as B. longum, one child from patients group contained species B. dentium and one baby B. adolescentis. Our results suggest that there are significantly lower counts of bifidobacteria in infants with allergic colitis than in healthy infants.
- MeSH
- alergie mikrobiologie MeSH
- anaerobní bakterie klasifikace genetika izolace a purifikace MeSH
- Bifidobacterium klasifikace genetika izolace a purifikace MeSH
- feces mikrobiologie MeSH
- grampozitivní bakterie klasifikace genetika izolace a purifikace MeSH
- hybridizace in situ fluorescenční MeSH
- kojenec MeSH
- kojení MeSH
- kolitida mikrobiologie MeSH
- kultivační média MeSH
- lidé MeSH
- počet mikrobiálních kolonií MeSH
- střeva mikrobiologie MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- kultivační média MeSH
DNA fingerprinting methods, RAPD with 7 random primers, and rep-PCR using both BOXA1R and (GTG)(5) ones, were used for the discrimination of 16 type and collection Bifidobacterium strains of 9 species of human origin, B. animalis ssp. animalis and B. animalis ssp. lactis and 7 Bifidobacterium strains collected in the Culture Collection of Dairy Microorganisms (CCDM). Both RAPD and rep-PCR methods provided similar results. The strains were identified as B. animalis ssp. lactis (6 strains) and B. adolescentis (1 strain). The reclassification of the collection strain CCM 3761 as B. pseudocatenulatum species (previously classified as B. adolescentis) was confirmed.
- MeSH
- Bifidobacterium klasifikace genetika izolace a purifikace MeSH
- DNA bakterií genetika MeSH
- DNA fingerprinting metody MeSH
- fylogeneze MeSH
- lidé MeSH
- polymerázová řetězová reakce metody MeSH
- technika náhodné amplifikace polymorfní DNA metody MeSH
- techniky typizace bakterií metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA bakterií MeSH