Clinical evidence suggests that healing is faster and almost scarless at an early neonatal age in comparison with that in adults. In this study, the phenotypes of neonatal and adult dermal fibroblasts and keratinocytes (nestin, smooth muscle actin, keratin types 8, 14 and 19, and fibronectin) were compared. Furthermore, functional assays (proliferation, migration, scratch wound closure) including mutual epithelial‑mesenchymal interactions were also performed to complete the series of experiments. Positivity for nestin and α smooth muscle actin was higher in neonatal fibroblasts (NFs) when compared with their adult counterparts (adult fibroblasts; AFs). Although the proliferation of NFs and AFs was similar, they significantly differed in their migration potential. The keratinocyte experiments revealed small, poorly differentiated cells (positive for keratins 8, 14 and 19) in primary cultures isolated from neonatal tissues. Moreover, the neonatal keratinocytes exhibited significantly faster rates of healing the experimentally induced in vitro defects in comparison with adult cells. Notably, the epithelial/mesenchymal interaction studies showed that NFs in co-culture with adult keratinocytes significantly stimulated the adult epithelial cells to acquire the phenotype of small, non-confluent cells expressing markers of poor differentiation. These results indicate the important differences between neonatal and adult cells that may be associated with improved wound healing during the early neonatal period.

• MeSH
• Actins metabolism   MeSH
• Cell Differentiation MeSH
• Neural Crest cytology   MeSH
• Tissue Donors * MeSH
• Adult MeSH
• Epithelial Cells cytology   metabolism   MeSH
• Phenotype MeSH
• Fibroblasts cytology   metabolism   MeSH
• Fibronectins biosynthesis   MeSH
• Immunohistochemistry MeSH
• Keratinocytes cytology   metabolism   MeSH
• Stem Cells metabolism   MeSH
• Coculture Techniques MeSH
• Humans MeSH
• Mesoderm cytology   MeSH
• Myofibroblasts cytology   MeSH
• Nestin metabolism   MeSH
• Neuronal Plasticity MeSH
• Infant, Newborn MeSH
• Cell Movement MeSH
• Cell Proliferation MeSH
• Gene Expression Profiling MeSH
• Aging physiology   MeSH
• Gene Expression Regulation, Developmental MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Infant, Newborn MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• ACTA2 protein, human MeSH Browser
• Actins MeSH
• Fibronectins MeSH
• Nestin MeSH