The lipid phase of the uppermost human skin layer is thought to comprise highly rigid lipids in an orthorhombic phase state to protect the body against the environment. By synthesizing sphingosine-d28 deuterated N-lignoceroyl-d-erythro-sphingosine (ceramide [NS]), we compare the structure and dynamics of both chains of that lipid in biologically relevant mixtures using X-ray diffraction, 2 H NMR analysis, and infrared spectroscopy. Our results reveal a substantial fraction of sphingosine chains in a fluid and dynamic phase state at physiological temperature. These findings prompt revision of our current understanding of the skin lipid barrier, where an extended ceramide [NS] conformation is preferred and a possible domain structure is proposed. Mobile lipid chains may be crucial for skin elasticity and the translocation of physiologically important molecules.

• Klíčová slova
• NMR spectroscopy, chain conformation, lipids, membranes, nanostructure,
• MeSH
• ceramidy chemie   MeSH
• cholesterol chemie   MeSH
• deuterium chemie   MeSH
• kůže chemie   metabolismus   MeSH
• lidé MeSH
• magnetická rezonanční spektroskopie MeSH
• nanostruktury chemie   MeSH
• sfingosin chemie   MeSH
• spektrofotometrie infračervená MeSH
• teplota MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• ceramidy MeSH
• cholesterol MeSH
• deuterium MeSH
• sfingosin MeSH