Most cited article - PubMed ID 17571792
Antibody-quantum dot conjugates exhibit enhanced antibacterial effect vs. unconjugated quantum dots
DNA aptamers were developed against lipopolysaccharide (LPS) from E. coli O111:B4 and shown to bind both LPS and E. coli by a colorimetric enzyme-based microplate assay. The polyclonal aptamers were coupled to human C1qrs protein either directly using a bifunctional linker or indirectly using biotinylated aptamers and a streptavidin-C1qrs complex. Both systems significantly reduced colony counts when applied to E. coli O111:B4 and K12 strains across a series of 10x dilutions of the bacteria in the presence of human serum; it was diluted 1: 10(3) in order to avoid significant bacterial lysis by the competing alternate pathway of complement activation. A number of candidate DNA aptamer sequences were cloned and sequenced from the anti-LPS aptamer library for future screening of antibacterial or "antibiotic" potential and to aid in eventual development of an alternative therapy for antibiotic-resistant bacterial infections.
- MeSH
- Anti-Bacterial Agents chemistry immunology pharmacology MeSH
- SELEX Aptamer Technique MeSH
- Aptamers, Nucleotide chemistry genetics immunology pharmacology MeSH
- Escherichia coli drug effects immunology MeSH
- Escherichia coli Infections drug therapy immunology MeSH
- Complement C1 chemistry immunology MeSH
- Humans MeSH
- Lipopolysaccharides chemistry immunology MeSH
- Molecular Sequence Data MeSH
- Base Sequence MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Aptamers, Nucleotide MeSH
- Complement C1 MeSH
- Lipopolysaccharides MeSH