Vitamin D is a micronutrient with pleiotropic effects in humans. Due to sedentary lifestyles and increasing time spent indoors, a growing body of research is revealing that vitamin D deficiency is a global problem. Despite the routine measurement of vitamin D in clinical laboratories and many years of efforts, methods of vitamin D analysis have yet to be standardized and are burdened with significant difficulties. This review summarizes several key analytical and clinical challenges that accompany the current methods for measuring vitamin D. According to an external quality assessment, methods and laboratories still produce a high degree of variability. Structurally similar metabolites are a source of significant interference. Furthermore, there is still no consensus on the normal values of vitamin D in a healthy population. These and other problems discussed herein can be a source of inconsistency in the results of research studies.
- Klíčová slova
- LC–MS/MS, VDR, determination, genomic effects, metabolites, non-genomic effects, vitamin D,
- MeSH
- hodnocení stavu výživy * MeSH
- klinické laboratorní techniky metody normy MeSH
- laboratoře normy MeSH
- lidé MeSH
- nedostatek vitaminu D diagnóza MeSH
- referenční hodnoty MeSH
- referenční standardy MeSH
- reprodukovatelnost výsledků MeSH
- vitamin D analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- vitamin D MeSH
