Effects of ethanol inhibition, initial pH and buffering capacity of media on the catabolic activity of nongrowing cells of Candida utilis were studied. Effects of external conditions on the kinetic of ethanol oxidation and cell respiration are described by mathematical models. The results revealed a significant influence of both the external pH and the buffering capacity of the medium on the kinetic parameters of catabolic activity. The inhibitory effect results in the bottleneck of one of the reaction of the citrate cycle, glyoxylate cycle or electron transfer in a respiratory chain although the total rate of ethanol dissimilation increases under these conditions.

• MeSH
• aerobióza MeSH
• Candida účinky léků   metabolismus   MeSH
• energetický metabolismus MeSH
• ethanol metabolismus   MeSH
• kinetika MeSH
• koncentrace vodíkových iontů MeSH
• kultivační média farmakologie   MeSH
• oxidace-redukce MeSH
• pufry MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• ethanol MeSH
• kultivační média MeSH
• pufry MeSH

External pH affects the acidification induced by ethanol. The apparent specific rate of acidification depends on the dissociation properties and production of carbon dioxide, acetic and lactic acid which are intermediates of ethanol oxidation. The organic acids are transported by Nernst-Einstein diffusion. We designed and identified a new and simple mathematical model that allows us to describe the effect of external pH on nonstationary transport of dissociated intermediates of ethanol oxidation.

• MeSH
• acetáty metabolismus   MeSH
• aktivní transport MeSH
• biologické modely MeSH
• Candida metabolismus   MeSH
• draslík metabolismus   MeSH
• ethanol metabolismus   MeSH
• kinetika MeSH
• koncentrace vodíkových iontů MeSH
• kyselina mléčná MeSH
• kyselina octová MeSH
• laktáty metabolismus   MeSH
• oxid uhličitý metabolismus   MeSH
• oxidace-redukce MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• acetáty MeSH
• draslík MeSH
• ethanol MeSH
• kyselina mléčná MeSH
• kyselina octová MeSH
• laktáty MeSH
• oxid uhličitý MeSH