Nejvíce citovaný článek - PubMed ID 18481220
The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation
A quantitative fluorescent in situ hybridization method was employed to evaluate the competitive inhibitory effect of three Lactobacillus strains (Lactobacillus reuteri, Lactobacillus gasseri, and Lactobacillus plantarum) against Escherichia coli internalization in a model system of HT 29 cells. Furthermore, aggregation and adhesion abilities of the Lactobacillus strains were examined. All lactobacilli were able to attach to the HT 29 cells and aggregate with pathogens; however, the adhesion and aggregation degree was strain-dependent. L. reuteri possessed a high capacity of adhesion (6.80 ± 0.63; log CFU ± SEM per well), whereas lower capacities were expressed by L. gasseri (4.52 ± 0.55) and L. plantarum (4.90 ± 0.98). Additionally, L. reuteri showed the rapid or normal ability to aggregate with selected E. coli in comparison with remaining two lactobacilli, which showed only slow or negative aggregative reaction. Internalization of E. coli into the cell lines was markedly suppressed by L. reuteri, while L. gasseri and L. plantarum caused only a minimum anti-invasion effect. The fact that L. reuteri in our experiments showed an outstanding potential for adhering to the colon epithelial cell line, compared with the rest strains, suggested that one of the possible mechanisms of preventing pathogen adhesion and invasion is simple competitions at certain receptors and capability to block receptor binding sites, or that an avid interaction between L. reuteri and the host cell might be modulating intracellular events responsible for the E. coli internalization. Moreover, L. reuteri exhibited a strong ability to aggregate with E. coli, which could be another limiting factor of pathogen invasion.
The activity of antagonistic substances produced by Pseudomonas aeruginosa and Lactobacillus acidophilus against the planktonic and sessile populations of Staphylococcus aureus strains was demonstrated. The strongest effects were caused by probiotic L. acidophilus strain - bacteriocin-like inhibitory substances (BLIS) positive. However, the S. aureus A3 growth, adhesion and biofilm formation was also limited by cell-free supernatant of L. acidophilus H-1 (BLIS negative). Moreover, competitive direct interactions were observed between staphylococci and the above bacteria, which influenced the formation of dualspecies aggregates on the surface.
- MeSH
- antibakteriální látky metabolismus farmakologie MeSH
- antibióza MeSH
- bakteriální adheze účinky léků MeSH
- bakteriociny metabolismus farmakologie MeSH
- biofilmy účinky léků MeSH
- kultivační média speciální farmakologie MeSH
- Lactobacillus acidophilus růst a vývoj metabolismus fyziologie MeSH
- lidé MeSH
- plankton růst a vývoj MeSH
- probiotika MeSH
- Pseudomonas aeruginosa růst a vývoj metabolismus fyziologie MeSH
- Staphylococcus aureus účinky léků růst a vývoj fyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- bakteriociny MeSH
- kultivační média speciální MeSH
Three Lactobacillus strains (LOCK 0900, LOCK 0908, LOCK 0919) out of twenty-four isolates were selected according to their antagonistic activity against pathogenic bacteria, resistance to low pH and milieu of bile salts. Intragastric administration of a mixture of these strains to Balb/c mice affected cytokine T(H)1-T(H)2 balance toward nonallergic T(H)1 response. Spleen cells, isolated from lactobacilli-treated mice and re-stimulated in vitro with the mixture of heat-inactivated tested strains, produced significantly higher amounts of anti-allergic tumor necrosis factor- and interferon-gamma than control animals whereas the level of pro-allergic interleukin-5 was significantly lower. Lactobacillus cells did not translocate through the intestinal barrier into blood, liver and spleen; a few Lactobacillus cells found in mesenteric lymph nodes could create antigenic reservoir activating the immune system. The mixture of Lactobacillus LOCK 0900, LOCK 0908 and LOCK 0919 strains represents a probiotic bacterial preparation with possible use in prophylaxis and/or therapy of allergic diseases.
- MeSH
- antibakteriální látky farmakologie MeSH
- antibióza MeSH
- bakteriální translokace MeSH
- fyziologický stres MeSH
- interferon gama metabolismus MeSH
- interleukin-5 metabolismus MeSH
- játra mikrobiologie MeSH
- krev mikrobiologie MeSH
- kultivované buňky MeSH
- kyseliny farmakologie MeSH
- Lactobacillus účinky léků fyziologie MeSH
- leukocyty mononukleární imunologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- probiotika aplikace a dávkování farmakologie MeSH
- slezina imunologie mikrobiologie MeSH
- TNF-alfa metabolismus MeSH
- žlučové kyseliny a soli farmakologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- interferon gama MeSH
- interleukin-5 MeSH
- kyseliny MeSH
- TNF-alfa MeSH
- žlučové kyseliny a soli MeSH
A quantitative approach has been proposed to evaluate the competitive inhibition of Escherichia coli and Salmonella typhi by live and heat-inactivated laboratory isolated Lactobacillus sp. on adhesion to monolayer of Caco-2 cells. Three species of Lactobacillus (L. casei, L. acidophilus, L. agilis) isolated from human neonate feces and two commercial probiotic strains (L. casei, L. acidophilus) have been compared for probiotic activity. All lactobacilli were able to attach to the Caco-2 cells, however, the degree of adhesion was bacterial strain-dependent. The adhesion indices of the two commercial probiotic strains were not significantly different from the values obtained for the other two similar fecal strains (p > 0.01). The inhibition of attachment of the pathogenic bacteria by inactivated cells of fecal L. acidophilus was examined and compared to the results of live bacteria. The inhibition pattern was similar for live and heat-inactivated L. acidophilus (p > 0.01). The number of attached pathogenic bacteria to the Caco-2 cells decreased when the number of L. acidophilus increased from 10(6) to 10(9) CFU/mL. The heat-inactivated L. acidophilus displayed similar probiotic activity compared to the live bacteria.
- MeSH
- bakteriální adheze * MeSH
- Caco-2 buňky MeSH
- Escherichia coli fyziologie MeSH
- feces mikrobiologie MeSH
- Lactobacillus izolace a purifikace fyziologie MeSH
- lidé MeSH
- novorozenec MeSH
- probiotika izolace a purifikace MeSH
- Salmonella typhi fyziologie MeSH
- střeva mikrobiologie MeSH
- vysoká teplota MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
A group of 69 lactobacilli was isolated from caries lesions and root canals of early childhood caries (ECC) affected children treated in the Department of Pedodontics (Children's Teaching Hospital, Brno, Czech Republic). Biochemical and physiological properties of all strains were characterized by API 50 CH kit and conventional tube tests. The rep-PCR fingerprinting with the (GTG)(5) primer was used for genotypic grouping of the isolates. The (GTG)(5)-PCR fingerprinting grouped all analyzed strains into a few clusters in nearly full agreement with phenotype identification results and clarified the taxonomic position of 13 biochemically unidentified strains. In total, 20 strains of Lactobacillus fermentum, 17 L. rhamnosus, 14 L. casei/paracasei, 7 L. gasseri, 7 L. salivarius and 4 L. plantarum were identified. Mixtures of two or even three Lactobacillus spp. were isolated from a few root canal content samples. Results obtained by biotyping and (GTG)(5)-PCR were generally comparable except for L. gasseri strains that were not biochemically identified. The (GTG)(5)-PCR fingerprinting was shown to be quicker, easier to perform and more reliable than biotyping. Our results imply this molecular method as a good tool for screening and identification of Lactobacillus spp. inhabiting dental plaque.
- MeSH
- dítě MeSH
- DNA fingerprinting MeSH
- DNA primery genetika MeSH
- fylogeneze MeSH
- Lactobacillus klasifikace genetika izolace a purifikace MeSH
- lidé MeSH
- techniky typizace bakterií MeSH
- zubní kaz mikrobiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA primery MeSH
Twelve lactobacilli isolates from mucosa of 3-5-week-old weaned pigs were found to exert good antimicrobial activity against common porcine pathogens (S. aureus, B. cereus, E. coli, C. perfringens). Two of them produced in addition to lactic acid also considerable amounts of acetic acid, and 6 of them produced hydrogen peroxide and metabolites other than organic acids. Isolates 4/26 and 2/25 (identified as L. crispatus or L. amylovorus) were inhibitory against most strains of S. aureus, B. cereus and E. coli, and especially the strain 4/26 survived well in simulated gastric and intestinal juice. Diarrhea-causing E. coli O8K88H9 Ent(+) was successfully inhibited by the growing culture as well as by the catalase-treated and neutralized supernatant of L. reuteri 12/26. Mucin degradation and multiple resistance to antibiotics were not observed.
- MeSH
- antibiotická rezistence MeSH
- Bacillus cereus účinky léků MeSH
- Clostridium perfringens účinky léků MeSH
- Escherichia coli účinky léků MeSH
- feces mikrobiologie MeSH
- ileum mikrobiologie MeSH
- kultivační média speciální farmakologie MeSH
- kultivační média chemie farmakologie MeSH
- kyselina mléčná farmakologie MeSH
- Lactobacillus účinky léků izolace a purifikace metabolismus MeSH
- muciny metabolismus MeSH
- nemoci prasat prevence a kontrola MeSH
- odstavení MeSH
- peroxid vodíku metabolismus MeSH
- přirozená imunita MeSH
- probiotika MeSH
- Staphylococcus aureus účinky léků MeSH
- střevní sliznice mikrobiologie MeSH
- Sus scrofa mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kultivační média speciální MeSH
- kultivační média MeSH
- kyselina mléčná MeSH
- muciny MeSH
- peroxid vodíku MeSH
