Calorie restriction-induced weight loss is accompanied by profound changes in adipose tissue characteristics. To determine the effect of weight loss on differentiation of preadipocytes and secretory capacity of in vitro differentiated adipocytes, we established cultures of these cells from paired subcutaneous adipose tissue biopsies obtained before and at the end of weight-reducing dietary intervention (DI) in 23 obese women. Based on lipid accumulation and the expression of differentiation markers, in vitro adipogenesis increased after weight loss and it was accompanied by enhanced expression of genes involved in de novo lipogenesis. This effect of weight loss was not driven by changes of peroxisome proliferator-activated receptor γ sensitivity to rosiglitazone. Weight loss also enhanced the expression of adiponectin and leptin while reducing that of monocyte chemoattractant protein 1 and interleukin-8 by cultured adipocytes. Thus, the weight-reducing (DI) increased adipogenic capacity of preadipocytes and shifted their secretion toward lower inflammatory profile. Reprogramming of preadipocytes could represent an adaptation to weight loss leading to partial restoration of preobese adipose tissue traits and thus contribute to the improvement of metabolic status. However, enhanced adipogenesis could also contribute to the unwanted weight regain after initial weight loss.

• MeSH
• Adipogenesis genetics   physiology   MeSH
• Adiponectin metabolism   MeSH
• Chemokine CCL2 metabolism   MeSH
• Cytokines metabolism   MeSH
• Enzyme-Linked Immunosorbent Assay MeSH
• Weight Loss genetics   physiology   MeSH
• Interleukin-8 metabolism   MeSH
• Cells, Cultured MeSH
• Leptin metabolism   MeSH
• Humans MeSH
• Obesity MeSH
• PPAR gamma metabolism   MeSH
• Rosiglitazone MeSH
• Thiazolidinediones pharmacology   MeSH
• Adipocytes cytology   MeSH
• Check Tag
• Humans MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Adiponectin MeSH
• Chemokine CCL2 MeSH
• Cytokines MeSH
• Interleukin-8 MeSH
• Leptin MeSH
• PPAR gamma MeSH
• Rosiglitazone MeSH
• Thiazolidinediones MeSH