Coeliac disease is a human, genetically linked, disorder which develops in gluten-sensitive persons. The aim of this study was to investigate the effect of prolonged feeding of gliadin, a major fraction of gluten, on enzyme activities of enterocyte brush border membrane enzymes in rats, mice and pigs. Brush-border membranes were isolated from mucosal scrapings of the small intestine of 21-d-old rat pups hand-fed with formula milk diet, two-month-old nu/nu and +/+ BALB/c mice and two-month-old piglets fed three times a week starting at birth with high doses of gliadin. Activities of lactase, sucrase and dipeptidyl peptidase IV (DPP IV) were determined. Individual animal models differed in their response to gliadin feeding. In comparison with albumin fed controls the activities of DPP IV and lactase were decreased in rat pups, nu/nu BALB/c mice and piglets. DPP IV activity was mostly affected in the ileum of rats and piglets fed with gliadin starting at birth. On the other hand, lactase and sucrase activities of nu/nu BALB/c mice and piglets decreased to the largest extent in jejunum.

• MeSH
• beta-galaktosidasa metabolismus   MeSH
• časové faktory MeSH
• celiakie enzymologie   MeSH
• dipeptidylpeptidasa 4 metabolismus   MeSH
• gliadin aplikace a dávkování   MeSH
• krysa rodu Rattus MeSH
• laktasa MeSH
• lidé MeSH
• mikroklky enzymologie   MeSH
• modely nemocí na zvířatech * MeSH
• myši inbrední BALB C MeSH
• myši nahé MeSH
• myši MeSH
• potkani Wistar MeSH
• prasata MeSH
• sacharasa metabolismus   MeSH
• střevní sliznice enzymologie   MeSH
• tenké střevo enzymologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• beta-galaktosidasa MeSH
• dipeptidylpeptidasa 4 MeSH
• gliadin MeSH
• laktasa MeSH
• sacharasa MeSH