Genes of the threonine operon of Escherichia coli were used for the construction of a Brevibacterium flavum strain excreting threonine. Using the shuttle vector pCEM300 and a newly constructed shuttle vector pEC71 (7.1 kb, Kmr/Nmr), various plasmids carrying E. coli thr genes were prepared. Mutants resistant to the threonine analog 2-amino-3-hydroxyvaleric acid (AHV) were isolated after the ethyl methanesulfonate treatment of B. flavum carrying these recombinant plasmids. A mutant of B. flavum CCM 351 carrying the cloned genes thrA and thrB accumulated 12 g/L of threonine after 48 h of cultivation.

• MeSH
• Genes, Bacterial genetics   MeSH
• Brevibacterium enzymology   genetics   metabolism   MeSH
• Escherichia coli genetics   MeSH
• Gene Expression physiology   MeSH
• Phosphotransferases (Alcohol Group Acceptor) metabolism   MeSH
• Genetic Vectors MeSH
• Homoserine Dehydrogenase metabolism   MeSH
• Lysine biosynthesis   MeSH
• Operon MeSH
• Plasmids MeSH
• Threonine biosynthesis   genetics   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Phosphotransferases (Alcohol Group Acceptor) MeSH
• Homoserine Dehydrogenase MeSH
• homoserine kinase MeSH Browser
• Lysine MeSH
• Threonine MeSH