A biosensor for the detection of hepatitis B antibodies in clinical saliva was developed. Compared to conventional analysis of blood serum, it offers the advantage of noninvasive collection of samples. Detection of biomarkers in saliva imposes two major challenges associated with the low analyte concentration and increased surface fouling. The detection of minute amounts of hepatitis B antibodies was performed by plasmonically amplified fluorescence sandwich immunoassay. To have access to specific detection, we prevented the nonspecific adsorption of biomolecules present in saliva by brushes of poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] grafted from the gold sensor surface and post modified with hepatitis B surface antigen. Obtained results were validated against the response measured with ELISA at a certified laboratory using serum from the same patients.

• MeSH
• biologické markery analýza   MeSH
• biosenzitivní techniky metody   MeSH
• fluorescenční spektrometrie MeSH
• hepatitida B - antigeny povrchové chemie   imunologie   MeSH
• hepatitida B - protilátky analýza   krev   imunologie   MeSH
• imobilizované proteiny chemie   imunologie   MeSH
• imunoanalýza MeSH
• lidé MeSH
• polymery chemie   MeSH
• povrchová plasmonová rezonance MeSH
• sliny metabolismus   MeSH
• zlato chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• hepatitida B - antigeny povrchové MeSH
• hepatitida B - protilátky MeSH
• imobilizované proteiny MeSH
• polymery MeSH
• zlato MeSH