A biosensor for the detection of hepatitis B antibodies in clinical saliva was developed. Compared to conventional analysis of blood serum, it offers the advantage of noninvasive collection of samples. Detection of biomarkers in saliva imposes two major challenges associated with the low analyte concentration and increased surface fouling. The detection of minute amounts of hepatitis B antibodies was performed by plasmonically amplified fluorescence sandwich immunoassay. To have access to specific detection, we prevented the nonspecific adsorption of biomolecules present in saliva by brushes of poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] grafted from the gold sensor surface and post modified with hepatitis B surface antigen. Obtained results were validated against the response measured with ELISA at a certified laboratory using serum from the same patients.

Biosensor Technologies AIT Austrian Institute of Technology GmbH Muthgasse 11 1190 Vienna Austria

DWI Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry RWTH Aachen University Forckenbeckstraße 50 52074 Aachen Germany

Institute of Macromolecular Chemistry AS CR v v i Heyrovsky Sq 2 162 06 Prague 6 Czech Republic

Molecular Diagnostics Health and Environment Department AIT Austrian Institute of Technology GmbH Muthgasse 11 1190 Vienna Austria

Zobrazit více v PubMed

Lee Y.-H.; Wong D. T. Am. J. Dent. 2009, 22, 241–248. PubMed PMC

Rehak N. N.; Cecco S. A.; Csako G. Clin. Chem. Lab. Med. 2000, 38, 335–343. 10.1515/CCLM.2000.049. PubMed DOI

Patil P. B.; Patil B. R. J. Indian Soc. Periodontol. 2011, 15, 310–317. 10.4103/0972-124X.92560. PubMed DOI PMC

Rathnayake N.; Åkerman S.; Klinge B.; Lundegren N.; Jansson H.; Tryselius Y.; Sorsa T.; Gustafsson A. PLoS One 2013, 8, e61356.10.1371/journal.pone.0061356. PubMed DOI PMC

Gitlin N. Clinical Chemistry 1997, 43, 1500–1506. PubMed

Schweitzer A.; Horn J.; Mikolajczyk R. T.; Krause G.; Ott J. J. Lancet 2015, 386, 1546–1555. 10.1016/S0140-6736(15)61412-X. PubMed DOI

Lozano R.; Naghavi M.; Foreman K.; Lim S.; Shibuya K.; Aboyans V.; Abraham J.; Adair T.; Aggarwal R.; Ahn S. Y.; et al. Lancet 2012, 380, 2095–2128. 10.1016/S0140-6736(12)61728-0. PubMed DOI PMC

Noppornpanth S.; Sathirapongsasuti N.; Chongsrisawat V.; Poovorawan Y. Southeast Asian J. Trop. Med. Public Health 2000, 31, 419–421. PubMed

Brandtzaeg P. Ann. N. Y. Acad. Sci. 2007, 1098, 288–311. 10.1196/annals.1384.012. PubMed DOI

Patidar K. A.; Parwani R. N.; Wanjari S. P. J. Oral Sci. 2011, 53, 97–102. 10.2334/josnusd.53.97. PubMed DOI

Chiappin S.; Antonelli G.; Gatti R.; De Palo E. F. Clin. Chim. Acta 2007, 383, 30–40. 10.1016/j.cca.2007.04.011. PubMed DOI

Homola J. Chem. Rev. 2008, 108, 462–493. 10.1021/cr068107d. PubMed DOI

Stewart M. E.; Anderton C. R.; Thompson L. B.; Maria J.; Gray S. K.; Rogers J. A.; Nuzzo R. G. Chem. Rev. 2008, 108, 494–521. 10.1021/cr068126n. PubMed DOI

Khatua S.; Paulo P. M.; Yuan H.; Gupta A.; Zijlstra P.; Orrit M. ACS Nano 2014, 8, 4440–4449. 10.1021/nn406434y. PubMed DOI

Liebermann T.; Knoll W. Colloids Surf., A 2000, 171, 115–130. 10.1016/S0927-7757(99)00550-6. DOI

Lakowicz J. R.; Ray K.; Chowdhury M.; Szmacinski H.; Fu Y.; Zhang J.; Nowaczyk K. Analyst 2008, 133, 1308–1346. 10.1039/b802918k. PubMed DOI PMC

Choi Y.-H.; Lee G.-Y.; Ko H.; Chang Y. W.; Kang M.-J.; Pyun J.-C. Biosens. Bioelectron. 2014, 56, 286–294. 10.1016/j.bios.2014.01.035. PubMed DOI

Zheng S.; Kim D.-K.; Park T. J.; Lee S. J.; Lee S. Y. Talanta 2010, 82, 803–809. 10.1016/j.talanta.2010.05.059. PubMed DOI

Helton K. L.; Nelson K. E.; Fu E.; Yager P. Lab Chip 2008, 8, 1847.10.1039/b811150b. PubMed DOI

Blaszykowski C.; Sheikh S.; Thompson M. Biomater. Sci. 2015, 3, 1335–1370. 10.1039/C5BM00085H. PubMed DOI

Chung J. W.; Kim S. D.; Bernhardt R.; Pyun J. C. Sens. Actuators, B 2005, 111–112, 416–422. 10.1016/j.snb.2005.03.055. DOI

Rodriguez-Emmenegger C.; Hasan E.; Pop-Georgievski O.; Houska M.; Brynda E.; Alles A. B. Macromol. Biosci. 2012, 12, 525–532. 10.1002/mabi.201100425. PubMed DOI

Riedel T.; Surman F.; Hageneder S.; Pop-Georgievski O.; Noehammer C.; Hofner M.; Brynda E.; Rodriguez-Emmenegger C.; Dostálek J. Biosens. Bioelectron. 2016, 85, 272–279. 10.1016/j.bios.2016.05.014. PubMed DOI

Jones D. M.; Brown A. A.; Huck W. T. S. Langmuir 2002, 18, 1265–1269. 10.1021/la011365f. DOI

Rodriguez-Emmenegger C.; Schmidt B. V.; Sedlakova Z.; Subr V.; Alles A. B.; Brynda E.; Barner-Kowollik C. Macromol. Rapid Commun. 2011, 32, 958–965. 10.1002/marc.201100176. PubMed DOI

Rodriguez-Emmenegger C.; Houska M.; Alles A. B.; Brynda E. Macromol. Biosci. 2012, 12, 1413–1422. 10.1002/mabi.201200171. PubMed DOI

Ulbrich K.; Šubr V.; Strohalm J.; Plocová D.; Jelínková M.; Říhová B. J. Controlled Release 2000, 64, 63–79. 10.1016/S0168-3659(99)00141-8. PubMed DOI

Hermanson G. T. In Bioconjugate Techniques, 3rd ed.; Academic Press: Boston, 2013; pp 229–258.

Lee Y. S.; Kim B. K.; Choi E. C. Arch. Pharmacal Res. 1998, 21, 521–526. 10.1007/BF02975368. PubMed DOI

Vaisocherová-Lísalová H.; Surman F.; Víšová I.; Vala M.; Špringer T.; Ermini M. L.; Šípová H.; Šedivák P.; Houska M.; Riedel T. s.; et al. Anal. Chem. 2016, 88, 10533–10539. 10.1021/acs.analchem.6b02617. PubMed DOI

Vaisocherová H.; Ševců V.; Adam P.; Špačková B.; Hegnerová K.; de los Santos Pereira A.; Rodriguez-Emmenegger C.; Riedel T.; Houska M.; Brynda E.; Homola J. Biosens. Bioelectron. 2014, 51, 150–157. 10.1016/j.bios.2013.07.015. PubMed DOI

Bauch M.; Dostalek J. Opt. Express 2013, 21, 20470–20483. 10.1364/OE.21.020470. PubMed DOI

Wang Y.; Brunsen A.; Jonas U.; Dostálek J.; Knoll W. Anal. Chem. 2009, 81, 9625–9632. 10.1021/ac901662e. PubMed DOI

Toma K.; Adam P.; Vala M.; Homola J.; Knoll W.; Dostalek J. Opt. Express 2013, 21, 10121–10132. 10.1364/OE.21.010121. PubMed DOI