A biosensor for the detection of hepatitis B antibodies in clinical saliva was developed. Compared to conventional analysis of blood serum, it offers the advantage of noninvasive collection of samples. Detection of biomarkers in saliva imposes two major challenges associated with the low analyte concentration and increased surface fouling. The detection of minute amounts of hepatitis B antibodies was performed by plasmonically amplified fluorescence sandwich immunoassay. To have access to specific detection, we prevented the nonspecific adsorption of biomolecules present in saliva by brushes of poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] grafted from the gold sensor surface and post modified with hepatitis B surface antigen. Obtained results were validated against the response measured with ELISA at a certified laboratory using serum from the same patients.

Biosensor Technologies AIT Austrian Institute of Technology GmbH Muthgasse 11 1190 Vienna Austria

DWI Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry RWTH Aachen University Forckenbeckstraße 50 52074 Aachen Germany

Institute of Macromolecular Chemistry AS CR v v i Heyrovsky Sq 2 162 06 Prague 6 Czech Republic

Molecular Diagnostics Health and Environment Department AIT Austrian Institute of Technology GmbH Muthgasse 11 1190 Vienna Austria

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