Chickens represent one of the most important sources of animal protein for the human population. However, chickens also represent one of the most important reservoirs of Salmonella for humans. Measures to decrease the Salmonella incidence in chickens are therefore continuously sought. In this study, we tested feed supplementation with a mixture of C1 to C12 monoacylglycerides. At 0.7 and 1.5 kg per ton of feed, such supplementation significantly decreased Salmonella counts in the caecum but not in the liver. The chickens were infected on day 4 and the protective effect in the caecum was recorded on day 22 and 23 of life. Supplementation also decreased the inflammatory response of chickens to Salmonella infection determined by avidin, SAA, ExFABP, MMP7, IL1β, IL4I and MRP126 gene expression but did not affect immunoglobulin expression in the caecum. C1 to C12 monoacylglycerides can be used as a feed supplement which, if continuously provided in feed, decrease Salmonella counts in chickens just prior slaughter.

• Klíčová slova
• Caecum, Chicken, Monoacylglyceride, Salmonella,
• MeSH
• cékum mikrobiologie   MeSH
• dieta veterinární   MeSH
• krmivo pro zvířata analýza   MeSH
• kur domácí * MeSH
• monoglyceridy * aplikace a dávkování   metabolismus   MeSH
• náhodné rozdělení MeSH
• nemoci drůbeže * imunologie   mikrobiologie   prevence a kontrola   MeSH
• odolnost vůči nemocem * účinky léků   MeSH
• potravní doplňky analýza   MeSH
• Salmonella enteritidis * fyziologie   MeSH
• salmonelová infekce u zvířat * imunologie   mikrobiologie   prevence a kontrola   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• monoglyceridy * MeSH

Clostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in vitro selective growth-inhibitory effect of 8-hydroxyquinoline (8HQ) on C. perfringens vs. bifidobacteria in a medium containing chicken ileal digesta. Prior to the experiments, the minimum inhibitory concentrations of 8HQ and penicillin G were determined by broth microdilution assay. The minimum inhibitory concentration values of 8HQ for C. perfringens were 16-32 times lower than the values for bifidobacteria. Treatment of autoclaved and non-autoclaved chicken ileal digesta with 8HQ showed a selective anticlostridial effect. After incubation of C. perfringens with autoclaved ileal digesta for 3 h, all 8HQ concentrations tested (32-2048 μg/mL) significantly reduced C. perfringens bacterial count. In contrast, the same treatment had no or only a slight effect on bifidobacteria counts. Unlike 8HQ, penicillin G did not exhibit any selectivity. Similar results were obtained after incubation for 24 h. In non-autoclaved ileal digesta, all 8HQ concentrations tested significantly reduced C. perfringens bacterial counts after incubation for 30 min and 3 h, while no effect was observed on bifidobacteria. These results suggest that 8HQ may serve as a prospective veterinary compound for use against necrotic enteritis in poultry.

• MeSH
• antibakteriální látky farmakologie   MeSH
• Bifidobacterium účinky léků   MeSH
• Clostridium perfringens účinky léků   MeSH
• ileum mikrobiologie   MeSH
• klostridiové infekce prevence a kontrola   veterinární   virologie   MeSH
• kur domácí mikrobiologie   MeSH
• nemoci drůbeže prevence a kontrola   virologie   MeSH
• oxychinolin farmakologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• oxychinolin MeSH