Diamond-like carbon (DLC) thin films are promising for use in coating orthopaedic, dental and cardiovascular implants. The problem of DLC layers lies in their weak layer adhesion to metal implants. Chromium is used as a dopant for improving the adhesion of DLC films. Cr-DLC layers were prepared by a hybrid technology, using a combination of pulsed laser deposition (PLD) from a graphite target and magnetron sputtering. Depending on the deposition conditions, the concentration of Cr in the DLC layers moved from zero to 10.0 at.%. The effect of DLC layers with 0.0, 0.9, 1.8, 7.3, 7.7 and 10.0 at.% Cr content on the adhesion and osteogenic differentiation of human osteoblast-like Saos-2 cells was assessed in vitro. The DLC samples that contained 7.7 and 10.0 at.% of Cr supported cell spreading on day 1 after seeding. On day three after seeding, the most apparent vinculin-containing focal adhesion plaques were also found on samples with higher concentrations of chromium. On the other hand, the expression of type I collagen and alkaline phosphatase at the mRNA and protein level was the highest on Cr-DLC samples with a lower concentration of Cr (0-1.8 at.%). We can conclude that higher concentrations of chromium supported cell adhesion; however DLC and DLC doped with a lower concentration of chromium supported osteogenic cell differentiation.

• MeSH
• Alkaline Phosphatase metabolism   MeSH
• Coated Materials, Biocompatible MeSH
• Cell Adhesion * MeSH
• Cell Differentiation * MeSH
• Cell Line MeSH
• Chromium chemistry   MeSH
• Diamond chemistry   MeSH
• Focal Adhesions MeSH
• Collagen Type I metabolism   MeSH
• Metals chemistry   MeSH
• Lasers MeSH
• Humans MeSH
• RNA, Messenger metabolism   MeSH
• Osteoblasts cytology   MeSH
• Osteogenesis MeSH
• Surface Properties MeSH
• Gene Expression Profiling MeSH
• Talin chemistry   MeSH
• Carbon chemistry   MeSH
• Vinculin metabolism   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Alkaline Phosphatase MeSH
• Coated Materials, Biocompatible MeSH
• Chromium MeSH
• Diamond MeSH
• Collagen Type I MeSH
• Metals MeSH
• RNA, Messenger MeSH
• Talin MeSH
• Carbon MeSH
• Vinculin MeSH

Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

• Keywords
• Saos-2 cells, cell adhesion, nanostructure, osteogenic differentiation, titanium nanotubes,
• MeSH
• Actins metabolism   MeSH
• Biomarkers metabolism   MeSH
• Cell Adhesion drug effects   MeSH
• Cell Differentiation drug effects   MeSH
• Fluorescent Antibody Technique MeSH
• Photoelectron Spectroscopy MeSH
• Humans MeSH
• Cell Line, Tumor MeSH
• Nanotubes chemistry   MeSH
• Osteoblasts cytology   drug effects   MeSH
• Osteogenesis drug effects   MeSH
• Surface Properties MeSH
• Cell Proliferation drug effects   MeSH
• Alloys MeSH
• Titanium pharmacology   MeSH
• Particle Size * MeSH
• Cell Survival drug effects   MeSH
• Vinculin metabolism   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Actins MeSH
• Biomarkers MeSH
• Alloys MeSH
• Titanium MeSH
• titanium alloy (TiAl6V4) MeSH Browser
• Vinculin MeSH

An investigation was made of the adhesion, growth and differentiation of osteoblast-like MG-63 and Saos-2 cells on titanium (Ti) and niobium (Nb) supports and on TiNb alloy with surfaces oxidized at 165°C under hydrothermal conditions and at 600°C in a stream of air. The oxidation mode and the chemical composition of the samples tuned the morphology, topography and distribution of the charge on their surfaces, which enabled us to evaluate the importance of these material characteristics in the interaction of the cells with the sample surface. Numbers of adhered MG-63 and Saos-2 cells correlated with the number of positively-charged (related with the Nb2O5 phase) and negatively-charged sites (related with the TiO2 phase) on the alloy surface. Proliferation of these cells is correlated with the presence of positively-charged (i.e. basic) sites of the Nb2O5 alloy phase, while cell differentiation is correlated with negatively-charged (acidic) sites of the TiO2 alloy phase. The number of charged sites and adhered cells was substantially higher on the alloy sample oxidized at 600°C than on the hydrothermally treated sample at 165°C. The expression values of osteoblast differentiation markers (collagen type I and osteocalcin) were higher for cells grown on the Ti samples than for those grown on the TiNb samples. This was more particularly apparent in the samples treated at 165°C. No considerable immune activation of murine macrophage-like RAW 264.7 cells on the tested samples was found. The secretion of TNF-α by these cells into the cell culture media was much lower than for either cells grown in the presence of bacterial lipopolysaccharide, or untreated control samples. Thus, oxidized Ti and TiNb are both promising materials for bone implantation; TiNb for applications where bone cell proliferation is desirable, and Ti for induction of osteogenic cell differentiation.

• MeSH
• Biomarkers metabolism   MeSH
• Cell Adhesion drug effects   MeSH
• Cell Differentiation drug effects   MeSH
• Cell Line MeSH
• Collagen Type I metabolism   MeSH
• Humans MeSH
• Lipopolysaccharides pharmacology   MeSH
• Macrophages cytology   drug effects   metabolism   MeSH
• Mice MeSH
• Osteoblasts cytology   drug effects   metabolism   MeSH
• Osteocalcin metabolism   MeSH
• Oxidation-Reduction MeSH
• Surface Properties MeSH
• Cell Proliferation drug effects   MeSH
• Alloys chemistry   pharmacology   MeSH
• Static Electricity MeSH
• Tissue Scaffolds * MeSH
• Tumor Necrosis Factor-alpha pharmacology   MeSH
• Hot Temperature MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Biomarkers MeSH
• Collagen Type I MeSH
• Lipopolysaccharides MeSH
• Osteocalcin MeSH
• Alloys MeSH
• titanium-niobium alloy MeSH Browser
• Tumor Necrosis Factor-alpha MeSH