Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28000113
DOI
10.1007/s10856-016-5830-2
PII: 10.1007/s10856-016-5830-2
Knihovny.cz E-zdroje
- MeSH
- alkalická fosfatasa metabolismus MeSH
- biokompatibilní potahované materiály MeSH
- buněčná adheze * MeSH
- buněčná diferenciace * MeSH
- buněčné linie MeSH
- chrom chemie MeSH
- diamant chemie MeSH
- fokální adheze MeSH
- kolagen typu I metabolismus MeSH
- kovy chemie MeSH
- lasery MeSH
- lidé MeSH
- messenger RNA metabolismus MeSH
- osteoblasty cytologie MeSH
- osteogeneze MeSH
- povrchové vlastnosti MeSH
- stanovení celkové genové exprese MeSH
- talin chemie MeSH
- uhlík chemie MeSH
- vinkulin metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alkalická fosfatasa MeSH
- biokompatibilní potahované materiály MeSH
- chrom MeSH
- diamant MeSH
- kolagen typu I MeSH
- kovy MeSH
- messenger RNA MeSH
- talin MeSH
- uhlík MeSH
- vinkulin MeSH
Diamond-like carbon (DLC) thin films are promising for use in coating orthopaedic, dental and cardiovascular implants. The problem of DLC layers lies in their weak layer adhesion to metal implants. Chromium is used as a dopant for improving the adhesion of DLC films. Cr-DLC layers were prepared by a hybrid technology, using a combination of pulsed laser deposition (PLD) from a graphite target and magnetron sputtering. Depending on the deposition conditions, the concentration of Cr in the DLC layers moved from zero to 10.0 at.%. The effect of DLC layers with 0.0, 0.9, 1.8, 7.3, 7.7 and 10.0 at.% Cr content on the adhesion and osteogenic differentiation of human osteoblast-like Saos-2 cells was assessed in vitro. The DLC samples that contained 7.7 and 10.0 at.% of Cr supported cell spreading on day 1 after seeding. On day three after seeding, the most apparent vinculin-containing focal adhesion plaques were also found on samples with higher concentrations of chromium. On the other hand, the expression of type I collagen and alkaline phosphatase at the mRNA and protein level was the highest on Cr-DLC samples with a lower concentration of Cr (0-1.8 at.%). We can conclude that higher concentrations of chromium supported cell adhesion; however DLC and DLC doped with a lower concentration of chromium supported osteogenic cell differentiation.
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