Majority of Treponema pallidum ssp. pallidum MLST allelic profiles in the Czech Republic (2004-2022) belong to two SS14-like clusters
Jazyk angličtina Země Anglie, Velká Británie Médium electronic
Typ dokumentu časopisecké články
Grantová podpora
U19 AI144177
NIAID NIH HHS - United States
LX22NPO5103
National Institute of Virology and Bacteriology , Programme EXCELES
65269705
Ministerstvo Zdravotnictví Ceské Republiky
U19AI144177
US National Institutes of Health National Institute for Allergy and Infectious Disease
PubMed
39075238
PubMed Central
PMC11286942
DOI
10.1038/s41598-024-68656-5
PII: 10.1038/s41598-024-68656-5
Knihovny.cz E-zdroje
- MeSH
- alely * MeSH
- dospělí MeSH
- genotyp MeSH
- lidé středního věku MeSH
- lidé MeSH
- makrolidy farmakologie MeSH
- multilokusová sekvenční typizace * MeSH
- syfilis * mikrobiologie epidemiologie genetika MeSH
- Treponema pallidum * genetika izolace a purifikace MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- makrolidy MeSH
Syphilis is a multistage sexually transmitted disease caused by Treponema pallidum ssp. pallidum. In the Czech Republic, there are around 700-800 new syphilis cases annually, continuously increasing since 2012. This study analyzed a total of 1228 samples from 2004 to 2022. Of the PCR-positive typeable samples (n = 415), 68.7% were fully-typed (FT), and 31.3% were partially-typed. Most of the identified isolates belonged to the SS14-clade and only 6.3% were the Nichols-like cluster. While in the beginning of sample collection isolates have been macrolide-susceptible, recent isolates are completely resistant to macrolides. Among the FT samples, 34 different allelic profiles (APs) were found. Most of the profiles (n = 27) appeared just once in the Czech population, while seven profiles were detected more than twice. The most frequent APs belonged to two separate groups of SS14-like isolates, including group of 1.3.1 (ST 1) and 1.26.1 (ST 25) profiles, and the second group containing 1.1.8 (ST 3), 1.1.1 (ST 2), and 1.1.3 (ST 11) (representing 57.5%, and 25.3% of all detected APs, respectively). Both groups consistently differed in 6 nucleotide positions in five genes (TP0150, TP0324, TP0515, TP0548, and TP0691) coding amino-acid replacements suggesting that one or more of these differences could be involved in the higher success of the first group.
Department of Biology Faculty of Medicine Masaryk University Brno Czech Republic
Department of Dermatovenerology University Hospital Brno Czech Republic
Zobrazit více v PubMed
Global progress report on HIV, viral hepatitis and sexually transmitted infections, 2021—Accountability for the global health sector strategies 2016–2021: Actions for impact—World | ReliefWeb [Internet]. 2021 [cited 2023 Apr 20]. Available from: https://reliefweb.int/report/world/global-progress-report-hiv-viral-hepatitis-and-sexually-transmitted-infections-2021.
Global health sector strategy on Sexually Transmitted Infections, 2016–2021 [Internet]. [cited 2023 Apr 20]. Available from: https://www.who.int/publications/i/item/WHO-RHR-16.09.
Data provided by Dr. Hana Zákoucká, National Health Institute.
Stamm, L. V. & Bergen, H. L. A point mutation associated with bacterial macrolide resistance is present in both 23S rRNA genes of an erythromycin-resistant PubMed DOI PMC
Noda, A. A., Rodríguez, I. & Šmajs, D. Genotyping of PubMed DOI
Sweeney, E. L., Lowry, K., Seel, M. PubMed
Fernández-Naval, C. PubMed DOI
Pospíšilová, P. PubMed DOI PMC
Zondag, H. C. A. PubMed DOI PMC
Šmajs, D., Paštěková, L. & Grillová, L. Macrolide resistance in the Syphilis Spirochete, PubMed DOI PMC
Stamm, L. V., Stapleton, J. T. & Bassford, P. J. In vitro assay to demonstrate high-level erythromycin resistance of a clinical isolate of PubMed DOI PMC
Stamm, L. V. & Parrish, E. A. In-vitro activity of azithromycin and CP-63,956 against PubMed DOI
Šmajs, D., Mikalova, L., Strouhal, M.
R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria (2021). URL https://www.R-project.org/.
Tamura, K. & Nei, M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. PubMed
Kumar, S., Stecher, G. & Tamura, K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. PubMed DOI PMC
A new typing scheme demonstrates high discriminatory power for Treponema pallidum subspecies
