The actin family members, consisting of actin and actin-related proteins (ARPs), are essential components of chromatin remodeling complexes. ARP6, one of the nuclear ARPs, is part of the Snf-2-related CREB-binding protein activator protein (SRCAP) chromatin remodeling complex, which promotes the deposition of the histone variant H2A.Z into the chromatin. In this study, we showed that ARP6 influences the structure and the function of the nucleolus. ARP6 is localized in the central region of the nucleolus, and its knockdown induced a morphological change in the nucleolus. We also found that in the presence of high concentrations of glucose ARP6 contributed to the maintenance of active ribosomal DNA (rDNA) transcription by placing H2A.Z into the chromatin. In contrast, under starvation, ARP6 was required for cell survival through the repression of rDNA transcription independently of H2A.Z. These findings reveal novel pleiotropic roles for the actin family in nuclear organization and metabolic homeostasis.

• Keywords
• ARP6, Actin-related protein, Histone H2A.Z, Nucleolus, Wndchrm,
• MeSH
• Adenosine Triphosphatases metabolism   MeSH
• Actins metabolism   physiology   MeSH
• Cell Nucleolus metabolism   physiology   MeSH
• Chromosomal Proteins, Non-Histone metabolism   physiology   MeSH
• Transcription, Genetic physiology   MeSH
• Glucose metabolism   MeSH
• HeLa Cells MeSH
• Humans MeSH
• DNA, Ribosomal genetics   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Research Support, N.I.H., Intramural MeSH
• Names of Substances
• ACTR6 protein, human MeSH Browser
• Adenosine Triphosphatases MeSH
• Actins MeSH
• Chromosomal Proteins, Non-Histone MeSH
• Glucose MeSH
• DNA, Ribosomal MeSH
• SRCAP protein, human MeSH Browser