Nejvíce citovaný článek - PubMed ID 2276604
Protein phosphorylation in Streptomyces albus
The existence of phosphoprotein phosphatase (PPP) in aerial mycelium of S. granaticolor was demonstrated. Using inhibitors of serine and/or threonine PPP and specifically labeled substrate it was found that the PPP is of the serine and/or threonine type.
- MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- fosfoserin metabolismus MeSH
- fosfothreonin metabolismus MeSH
- inhibitory enzymů farmakologie MeSH
- proteinfosfatasy analýza antagonisté a inhibitory metabolismus MeSH
- Streptomyces enzymologie růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fosfoserin MeSH
- fosfothreonin MeSH
- inhibitory enzymů MeSH
- proteinfosfatasy MeSH
Using the examples of biosynthesis of streptomycin, bialaphos, actinorhodin, oligoketides and autoregulators during the first hours of streptomycete cultivation, it is stressed that the external environment in cooperation with the internal metabolic abilities of the cell determines the metabolic type that would develop during the life cycle of the producing streptomycetes. If we accept that a certain metabolic type (from the point of view of the production of secondary metabolites) was determined already during the first hours of cultivation of the microorganisms, we must also admit that the availability of primary metabolites in the so-called production phase of growth (stationary phase, idiophase, etc.) is to a certain extent determined by the very early stages of strain development.
- MeSH
- anthrachinony metabolismus MeSH
- antibakteriální látky biosyntéza MeSH
- časové faktory MeSH
- organofosforové sloučeniny metabolismus MeSH
- regulace genové exprese u bakterií MeSH
- Streptomyces fyziologie MeSH
- streptomycin biosyntéza MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- actinorhodin MeSH Prohlížeč
- anthrachinony MeSH
- antibakteriální látky MeSH
- bialaphos MeSH Prohlížeč
- organofosforové sloučeniny MeSH
- streptomycin MeSH
RNA polymerase was isolated from Streptomyces granaticolor and protein kinase was partially purified from Streptomyces albus. When RNA polymerase was treated with protein kinase in vitro the activity of RNA polymerase was markedly enhanced. Furthermore, a protein of M = 65 kDa was isolated which, after being phosphorylated, stimulated RNA polymerase activity in vitro. Because neither the beta-subunits nor the alpha-subunits of RNA polymerase were phosphorylated it is assumed that phosphorylation of the 65 kDa protein may regulate the activity of RNA polymerase in streptomycetes.
