Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals and cereal-based products. In addition to the regulated mycotoxins, for which official control is required, LC-MS is often used for the screening of a large range of mycotoxins and/or for the identification and characterization of novel metabolites. This review provides insight into the LC-MS methods used for the determination of co-occurring mycotoxins with special emphasis on multiple-analyte applications. The first part of the review is focused on targeted LC-MS approaches using cleanup methods such as solid-phase extraction and immunoaffinity chromatography, as well as on methods based on minimum cleanup (quick, easy, cheap, effective, rugged, and safe; QuEChERS) and dilute and shoot. The second part of the review deals with the untargeted determination of mycotoxins by LC coupled with high-resolution MS, which includes also metabolomics techniques to study the fate of mycotoxins in plants.

• Keywords
• Fungal secondary metabolites, Liquid chromatography–high-resolution mass spectrometry, Liquid chromatography–tandem mass spectrometry, Metabolomics, Validation,
• MeSH
• Food Analysis methods   MeSH
• Chromatography, Affinity methods   MeSH
• Solid Phase Extraction methods   MeSH
• Fungi isolation & purification   metabolism   MeSH
• Edible Grain chemistry   metabolism   microbiology   MeSH
• Metabolomics methods   MeSH
• Mycotoxins analysis   metabolism   MeSH
• Tandem Mass Spectrometry methods   MeSH
• Chromatography, High Pressure Liquid methods   MeSH
• Publication type
• Journal Article MeSH
• Review MeSH
• Names of Substances
• Mycotoxins MeSH