Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.

• Keywords
• N-myc, doxorubicin, ellipticine, etoposide, gold nanoparticles, liposome,
• MeSH
• DNA, Antisense chemistry   therapeutic use   MeSH
• Doxorubicin chemistry   therapeutic use   MeSH
• Ellipticines chemistry   therapeutic use   MeSH
• Etoposide chemistry   therapeutic use   MeSH
• Fluorescence MeSH
• Drug Delivery Systems * MeSH
• Humans MeSH
• Liposomes chemistry   therapeutic use   MeSH
• Magnetite Nanoparticles chemistry   therapeutic use   MeSH
• Neoplasms drug therapy   MeSH
• N-Myc Proto-Oncogene Protein antagonists & inhibitors   genetics   MeSH
• Gold chemistry   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• DNA, Antisense MeSH
• Doxorubicin MeSH
• Ellipticines MeSH
• Etoposide MeSH
• Liposomes MeSH
• Magnetite Nanoparticles MeSH
• N-Myc Proto-Oncogene Protein MeSH
• Gold MeSH