Bone and cartilage are tissues of a three-dimensional (3D) nature. Therefore, scaffolds for their regeneration should support cell infiltration and growth in all 3 dimensions. To fulfill such a requirement, the materials should possess large, open pores. Centrifugal spinning is a simple method for producing 3D fibrous scaffolds with large and interconnected pores. However, the process of bone regeneration is rather complex and requires additional stimulation by active molecules. In the current study, we introduced a simple composite scaffold based on platelet adhesion to poly-ε-caprolactone 3D fibers. Platelets were used as a natural source of growth factors and cytokines active in the tissue repair process. By immobilization in the fibrous scaffolds, their bioavailability was prolonged. The biological evaluation of the proposed system in the MG-63 model showed improved metabolic activity, proliferation and alkaline phosphatase activity in comparison to nonfunctionalized fibrous scaffold. In addition, the response of cells was dose dependent with improved biocompatibility with increasing platelet concentration. The results demonstrated the suitability of the system for bone tissue.

• Keywords
• 3D scaffold, PCL, centrifugal spinning, cytokines, growth factors, platelets,
• MeSH
• Platelet Adhesiveness drug effects   MeSH
• Alkaline Phosphatase metabolism   MeSH
• Kinetics MeSH
• Drug Delivery Systems methods   MeSH
• Humans MeSH
• Intercellular Signaling Peptides and Proteins administration & dosage   pharmacology   MeSH
• Cell Line, Tumor MeSH
• Osteoblasts cytology   drug effects   ultrastructure   MeSH
• Osteogenesis drug effects   MeSH
• Polyesters chemistry   pharmacology   MeSH
• Cell Proliferation drug effects   MeSH
• Tissue Engineering methods   MeSH
• Tissue Scaffolds chemistry   MeSH
• Blood Platelets drug effects   metabolism   ultrastructure   MeSH
• Cell Shape drug effects   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Alkaline Phosphatase MeSH
• Intercellular Signaling Peptides and Proteins MeSH
• polycaprolactone MeSH Browser
• Polyesters MeSH

Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

• Keywords
• growth factors, hernia regeneration, in vitro, nanofibers, polypropylene mesh,
• MeSH
• Biocompatible Materials * chemistry   toxicity   MeSH
• Surgical Mesh * MeSH
• Incisional Hernia surgery   MeSH
• Mice MeSH
• Nanofibers * chemistry   toxicity   MeSH
• Polyesters * chemistry   toxicity   MeSH
• Polypropylenes * chemistry   toxicity   MeSH
• Cell Proliferation drug effects   MeSH
• Blood Platelets cytology   MeSH
• Cell Survival drug effects   MeSH
• Animals MeSH
• Check Tag
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Biocompatible Materials * MeSH
• polycaprolactone MeSH Browser
• Polyesters * MeSH
• Polypropylenes * MeSH