Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

Department of Surgery 2nd Faculty of Medicine Charles University Prague Prague Czech Republic

Institute of Biophysics 2nd Faculty of Medicine Charles University Prague Prague Czech Republic ; Laboratory of Tissue Engineering Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic

Institute of Biophysics 2nd Faculty of Medicine Charles University Prague Prague Czech Republic ; Laboratory of Tissue Engineering Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic ; Faculty of Biomedical Engineering Czech Technical University Prague Kladno Czech Republic

Laboratory of Tissue Engineering Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic ; University Center for Energy Efficient Buildings The Czech Technical University Prague Bustehrad Czech Republic

University Center for Energy Efficient Buildings The Czech Technical University Prague Bustehrad Czech Republic

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