OBJECTIVES: The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell-seeded scaffold implants a one-step intervention. MATERIALS AND METHODS: We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro. Pig chondrocytes were seeded on the thrombocyte-coated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non-coated scaffolds. RESULTS: Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time-dependent degradation of thrombocytes and release of their GFs. CONCLUSIONS: These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.

Institute of Biophysics 2nd Faculty of Medicine Charles University Prague Prague Czech Republic

Zobrazit více v PubMed

Sampson S, Gerhardt M, Mandelbaum B (2008) Platelet rich plasma injection grafts for musculoskeletal injuries: a review. Curr. Rev. Musculoskelet. Med. 1, 165–174. PubMed PMC

Alsousou J, Thompson M, Hulley P, Noble A, Willett K (2009) The biology of platelet‐rich plasma and its application in trauma and orthopaedic surgery: a review of the literature. J. Bone Joint Surg. Br. 91, 987–996. PubMed

Hall MP, Band PA, Meislin RJ, Jazrawi LM, Cardone DA (2009) Platelet‐rich plasma: current concepts and application in sports medicine. J. Am. Acad. Orthop. Surg. 17, 602–608. PubMed

Kuznetsov SA, Mankani MH, Gronthos S, Satomura K, Bianco P, Robey PG (2001) Circulating skeletal stem cells. J. Cell Biol. 153, 1133–1140. PubMed PMC

Kajikawa Y, Morihara T, Watanabe N, Sakamoto H, Matsuda K, Kobayashi M et al. (2007) GFP chimeric models exhibited a biphasic pattern of mesenchymal cell invasion in tendon healing. J. Cell. Physiol. 210, 684–691. PubMed

Volkman A, Gowans JL (1965) The production of macrophages in the rat. Br. J. Exp. Pathol. 46, 50–61. PubMed PMC

Ma Z, Kotaki M, Inai R, Ramakrishna S (2005) Potential of nanofiber matrix as tissue‐engineering scaffolds. Tissue Eng. 11, 101–109. PubMed

Roldan JC, Jepsen S, Miller J, Freitag S, Rueger DC, Açil Y et al. (2004) Bone formation in the presence of platelet‐rich plasma vs. bone morphogenetic protein‐7. Bone 34, 80–90. PubMed

Lucarelli E, Fini M, Beccheroni A, Giavaresi G, Di Bella C, Aldini NN et al. (2005) Stromal stem cells and platelet‐rich plasma improve bone allograft integration. Clin. Orthop. Relat. Res. 435, 62–68. PubMed

Akeda K, An HS, Okuma M, Attawia M, Miyamoto K, Thonar EJ et al. (2006) Platelet‐rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis. Osteoarthritis Cartilage 14, 1272–1280. PubMed

Nagae M, Ikeda T, Mikami Y, Hase H, Ozawa H, Matsuda K et al. (2007) Intervertebral disc regeneration using platelet‐rich plasma and biodegradable gelatin hydrogel microspheres. Tissue Eng. 13, 147–158. PubMed

Anitua E, Sanchez M, Orivea G, Andia I (2007) The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials 28, 4551–4560. PubMed

Sanchez AR, Sheridan PJ, Kupp LI (2003) Is platelet‐rich plasma the perfect enhancement factor? A current review. Int. J. Oral Maxillofac. Implants 18, 93–103. PubMed

Everts PA, Brown Mahoney C, Hoffmann JJ, Schönberger JP, Box HA, van Zundert A et al. (2006) Platelet‐rich plasma preparation using three devices: implications for platelet activation and platelet growth factor release. Growth Factors 24, 165–171. PubMed

Kim TG, Park TG (2006) Surface functionalized electrospun biodegradable nanofibers for immobilization of bioactive molecules. Biotechnol. Prog. 22, 1108–1113. PubMed

Jayakumar R, Prabaharan M, Nair SV, Tamura H (2010) Novel chitin and chitosan nanofibers in biomedical applications. Biotechnol. Adv. 28, 142–150. PubMed

Hromadka M, Collins JB, Reed CH, Li K, Kamal K, Bruce A et al. (2008) Nanofiber applications for burn care. J. Burn Care Res. 29, 695–703. PubMed

Lukas D, Sarkar A, Martinova L, Vodsedalkova K, Lubasova D, Chaloupek J et al. (2009) Physical principles of electrospinning (electrospinning as a nano‐scale technology of the twenty‐first century). Textile Progress 41, 1–83.

Baenziger NL, Brodie GN, Majerus PW (1971) A thrombin‐sensitive protein of human platelet membranes. Proc. Natl. Acad. Sci. USA 68, 240–243. PubMed PMC

Ruzek D, Vancova M, Tesarova M, Ahantarig A, Kopecky J, Grubhoffer L (2009) Morphological changes in human neural cells following tick‐borne encephalitis virus infection. J. Gen. Virol. 90, 1649–1658. PubMed

Erlandsen SL, Ottenwaelter C, Fretham C, Chen Y (2001) Cryo field emission scanning electron microscopy. Biotechniques 31, 300–305. PubMed

Filova E, Rampichova M, Handl M, Lytvynets A, Halouzka R, Usvald D et al. (2007) Composite hyaluronate‐type I collagen‐fibrin scaffold in the therapy of osteochondral defects in miniature pigs. Physiol. Res. 56, 5–16. PubMed

Tvrdik D, Svatošová J, Dundr P, Povýšil C (2005) Molecular diagnosis of synovial sarcoma: detection of SYT‐SSX11/2 fusion transcripts by RT‐PCR in paraffin‐embedded tissue. Med. Sci. Monit. 11, MT1–MT7. PubMed

Yang H, Lang S, Zhai Z, Li L, Kahr WHA, Chen P et al. (2009) Fibrinogen is required for maintenance of platelet intracellular and cell‐surface P‐selectin expression. Blood 114, 425–436. PubMed

Bucala R, Spiegel LA, Chesney J, Hogan M, Cerami A (1994) Circulating fibrocytes define a new leukocyte subpopulation that mediates tissue repair. Mol. Med. 1, 71–81. PubMed PMC

Chesney J, Bucala R (1997) Peripheral blood fibrocytes: novel fibroblast‐like cells that present antigen and mediate tissue repair. Biochem. Soc. Trans. 25, 520–524. PubMed

Freymiller EG, Aghaloo TL (2004) Platelet‐rich plasma: ready or not? J. Oral Maxillofac. Surg. 62, 484–488. PubMed

Mishra A, Tummala P, King A, Lee B, Kraus M, Tse V et al. (2009) Buffered platelet‐rich plasma enhances mesenchymal stem cell proliferation and chondrogenic differentiation. Tissue Eng. Part C Methods 15, 431–435. PubMed PMC

Dumont LJ, VandenBroeke T (2003) Seven‐day storage of apheresis platelets: report of an in vitro study. Transfusion 43, 143–150. PubMed

Drengk A, Zapf A, Stürmer EK, Stürmer KM, Frosch KH (2009) Influence of platelet‐rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells. Cells Tissues Organs 189, 317–326. PubMed

Heywood HK, Lee DA (2008) Monolayer expansion induces an oxidative metabolism and ROS in chondrocytes. Biochem. Biophys. Res. Commun. 373, 224–229. PubMed

Tonti GA, Mannello F (2008) From bone marrow to therapeutic applications: different behaviour and genetic/epigenetic stability during mesenchymal stem cell expansion in autologous and foetal bovine sera? Int. J. Dev. Biol. 52, 1023–1032. PubMed

Liquid resorbable nanofibrous surgical mesh: a proof of a concept

A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment

Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits

Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells

Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution

Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-ε-caprolactone nanofibers and growth factors for prevention of incisional hernia formation

Thin-layer hydroxyapatite deposition on a nanofiber surface stimulates mesenchymal stem cell proliferation and their differentiation into osteoblasts