Fifteen vaccinia virus (VV) recombinants derived from VV strains Praha, LIVP and DD (i.e. Dryvax Wyeth vaccine-derived) and expressing genes for S, preS2-S or c antigens of hepatitis B virus (HBV) were tested in monkey CV-1 cells and human diploid LEP cells. The production of infectious virus was found to be alike in all the recombinants and parental viruses as well. However, several recombinants produced markedly lesser amounts of S and preS2 antigens in LEP cells than in CV-1 cells. This reduction was independent of the parental virus used. There was, however, a relationship between the production of preS2 in CV-1 cells and the production of S and preS2 antigens in LEP cells; in general, recombinants efficiently inducing preS2 antigen formation in CV-1 cells produced markedly reduced amounts of S and preS2 antigens in LEP cells. Reduction of HBV antigen production in LEP cells was not apparent in recombinants expressing only S or c antigens of HBV, and the production of c antigen by double recombinants was not influenced by simultaneous expression of preS2 and S. The various recombinants also differed in the ratio of S:preS2 antigen formation. This difference seemed to be associated with the length of the untranslated leader sequence preceding preS2 but not with the parental virus or cell type used. The titers of antibodies against S and preS2 antigens induced in mice immunized with different recombinants differed markedly. The differences in the ratio of S:preS2 antigen production in vitro were not reflected in vivo by S:preS2 antibody ratio.

• MeSH
• buněčné linie MeSH
• Haplorrhini MeSH
• hepatitida B - antigeny povrchové biosyntéza   genetika   imunologie   MeSH
• hepatitida B - antigeny biosyntéza   genetika   imunologie   MeSH
• klonování DNA MeSH
• kuřecí embryo MeSH
• lidé MeSH
• myši inbrední ICR MeSH
• myši MeSH
• proteinové prekurzory biosyntéza   genetika   imunologie   MeSH
• proteiny - lokalizační signály genetika   fyziologie   MeSH
• protilátky virové biosyntéza   MeSH
• rekombinantní proteiny biosyntéza   genetika   imunologie   MeSH
• virus vakcinie genetika   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• kuřecí embryo MeSH
• lidé MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• hepatitida B - antigeny povrchové MeSH
• hepatitida B - antigeny MeSH
• presurface protein 2, hepatitis B surface antigen MeSH Prohlížeč
• proteinové prekurzory MeSH
• proteiny - lokalizační signály MeSH
• protilátky virové MeSH
• rekombinantní proteiny MeSH

Five different recombinant vaccinia viruses expressing the envelope antigen of hepatitis B virus (HBsAg) under the control of the P7.5 promoter were constructed. Cell cultures infected with some of the recombinant viruses synthesized both middle (M) and major surface (S) protein of HBsAg. It was shown that the length of the nontranslated sequence preceding preS2-ATG influenced the extracellular or intracellular HBV antigen distribution and the preS2:S antigen ratio. Some recombinants synthesized an M protein that was enlarged by additional 35 amino acids of preS1 domain and was entirely retained within the infected cells. Antibody responses to the S and preS2 antigens in mice revealed significant differences in the immunogenicity of individual recombinants.

• MeSH
• buněčné linie MeSH
• DNA virů MeSH
• hepatitida B - antigeny povrchové biosyntéza   genetika   imunologie   MeSH
• imunizace MeSH
• klonování DNA MeSH
• krysa rodu Rattus MeSH
• molekulární sekvence - údaje MeSH
• myši MeSH
• peptidy genetika   MeSH
• plazmidy MeSH
• precipitinové testy MeSH
• proteinové prekurzory genetika   imunologie   MeSH
• proteiny - lokalizační signály genetika   imunologie   MeSH
• protilátky virové imunologie   MeSH
• regulace exprese virových genů MeSH
• sekvence nukleotidů MeSH
• Southernův blotting MeSH
• virus hepatitidy B genetika   imunologie   MeSH
• virus vakcinie genetika   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA virů MeSH
• hepatitida B - antigeny povrchové MeSH
• peptidy MeSH
• presurface protein 1, hepatitis B surface antigen MeSH Prohlížeč
• presurface protein 2, hepatitis B surface antigen MeSH Prohlížeč
• proteinové prekurzory MeSH
• proteiny - lokalizační signály MeSH
• protilátky virové MeSH

Recombinant vaccinia virus expressing the middle hepatitis B virus surface antigen was incapable of inducing marked antibody response against the S and pre-S2 antigenic specifities in mice. However, mice immunized with this virus produced antibodies to both these antigens after the following administration of subtreshold doses of plasmatic hepatitis B surface antigen.

• MeSH
• hepatitida B - antigeny povrchové genetika   imunologie   MeSH
• imunizace MeSH
• myši MeSH
• proteinové prekurzory genetika   imunologie   MeSH
• protilátky virové biosyntéza   MeSH
• virus vakcinie genetika   imunologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hepatitida B - antigeny povrchové MeSH
• presurface protein 2, hepatitis B surface antigen MeSH Prohlížeč
• proteinové prekurzory MeSH
• protilátky virové MeSH