Hepatitis B virus proteins expressed by recombinant vaccinia viruses: influence of preS2 sequence on expression surface and nucleocapsid proteins in human diploid cells
Language English Country Austria Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8279947
DOI
10.1007/bf01379102
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Haplorhini MeSH
- Hepatitis B Surface Antigens biosynthesis genetics immunology MeSH
- Hepatitis B Antigens biosynthesis genetics immunology MeSH
- Cloning, Molecular MeSH
- Chick Embryo MeSH
- Humans MeSH
- Mice, Inbred ICR MeSH
- Mice MeSH
- Protein Precursors biosynthesis genetics immunology MeSH
- Protein Sorting Signals genetics physiology MeSH
- Antibodies, Viral biosynthesis MeSH
- Recombinant Proteins biosynthesis genetics immunology MeSH
- Vaccinia virus genetics physiology MeSH
- Animals MeSH
- Check Tag
- Chick Embryo MeSH
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hepatitis B Surface Antigens MeSH
- Hepatitis B Antigens MeSH
- presurface protein 2, hepatitis B surface antigen MeSH Browser
- Protein Precursors MeSH
- Protein Sorting Signals MeSH
- Antibodies, Viral MeSH
- Recombinant Proteins MeSH
Fifteen vaccinia virus (VV) recombinants derived from VV strains Praha, LIVP and DD (i.e. Dryvax Wyeth vaccine-derived) and expressing genes for S, preS2-S or c antigens of hepatitis B virus (HBV) were tested in monkey CV-1 cells and human diploid LEP cells. The production of infectious virus was found to be alike in all the recombinants and parental viruses as well. However, several recombinants produced markedly lesser amounts of S and preS2 antigens in LEP cells than in CV-1 cells. This reduction was independent of the parental virus used. There was, however, a relationship between the production of preS2 in CV-1 cells and the production of S and preS2 antigens in LEP cells; in general, recombinants efficiently inducing preS2 antigen formation in CV-1 cells produced markedly reduced amounts of S and preS2 antigens in LEP cells. Reduction of HBV antigen production in LEP cells was not apparent in recombinants expressing only S or c antigens of HBV, and the production of c antigen by double recombinants was not influenced by simultaneous expression of preS2 and S. The various recombinants also differed in the ratio of S:preS2 antigen formation. This difference seemed to be associated with the length of the untranslated leader sequence preceding preS2 but not with the parental virus or cell type used. The titers of antibodies against S and preS2 antigens induced in mice immunized with different recombinants differed markedly. The differences in the ratio of S:preS2 antigen production in vitro were not reflected in vivo by S:preS2 antibody ratio.
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