We report a series of microarray-based leaf and crown transcriptome comparisons involving three barley cultivars (cvs. Luxor, Igri and Atlas 68) which express differing degrees of frost tolerance. The transcripts were obtained following the exposure of seedlings to low (above and below zero) temperatures, aiming to identify those genes and signalling/metabolic pathways which are associated with frost tolerance. Both the leaves and the crowns responded to low temperature by the up-regulation of a suite of abscisic acid (ABA)-responsive genes, most of which have already been recognized as components of the plant low temperature response. The inter-cultivar comparison indicated that genes involved in maintaining the leaf's capacity to synthesize protein and to retain chloroplast activity were important for the expression of frost tolerance. In the crown, the repression of genes associated with nucleosome assembly and transposon regulation were the most relevant transcriptional changes associated with frost tolerance, highlighting the role of gene repression in the cold acclimation response.

• MeSH
• down regulace MeSH
• ječmen (rod) genetika   metabolismus   MeSH
• listy rostlin genetika   metabolismus   MeSH
• mapování chromozomů MeSH
• nukleozomy genetika   metabolismus   MeSH
• reakce na chladový šok MeSH
• regulace genové exprese u rostlin MeSH
• rostlinné geny MeSH
• rostlinné proteiny genetika   metabolismus   MeSH
• transkripční elongační faktory genetika   metabolismus   MeSH
• transkriptom MeSH
• upregulace MeSH
• zmrazování MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• nukleozomy MeSH
• rostlinné proteiny MeSH
• transkripční elongační faktory MeSH