Nejvíce citovaný článek - PubMed ID 23990476
Rapid extraction of total RNA from an anaerobic sludge biocoenosis
Anaerobic microorganisms (anaerobes) possess a fascinating metabolic versatility. This characteristic makes anaerobes interesting candidates for physiological studies and utilizable as microbial cell factories. To investigate the physiological characteristics of an anaerobic microbial population, yield, productivity, specific growth rate, biomass production, substrate uptake, and product formation are regarded as essential variables. The determination of those variables in distinct cultivation systems may be achieved by using different techniques for sampling, measuring of growth, substrate uptake, and product formation kinetics. In this review, a comprehensive overview of methods is presented, and the applicability is discussed in the frame of anaerobic microbiology and biotechnology.
- Klíčová slova
- Archaea, Bacteria, Bioprocess, Cultivation, Microscopy, Molecular biology, Physiology, Process analytical technology,
- MeSH
- anaerobióza MeSH
- anaerobní bakterie růst a vývoj fyziologie MeSH
- biomasa MeSH
- bioreaktory mikrobiologie MeSH
- fermentace MeSH
- metabolické inženýrství metody MeSH
- průmyslová mikrobiologie metody MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH