There is a link between metabolism and reproduction as metabolic hormones affect hypothalamus-pituitary-testis (HPT) hormonal functions and vice versa. The aim of the present study was to investigate the effects of negative energy balance on the reproductive system in male goldfish exposed to testosterone (T) and 17β-estradiol (E2). Following 7 days of food deprivation (FD), ANOVA models showed significant FD × sex steroid interactions on sperm quality and circulating sex steroid levels. When FD effects were investigated, 11-ketotestosterone (11-KT) level and sperm motility and velocity decreased in food-deprived goldfish in the control group. In E2-exposed goldfish, FD decreased sperm production in addition to sperm motility and velocity that coincided with an elevation of circulating E2 level. However, FD did not significantly impact sex steroids and sperm quality in T-exposed goldfish. ANOVA models showed non-significant FD × sex steroid interactions for HSI, GSI, circulating luteinizing hormone (Lh) level, and metabolic (preproghrelin, goat and nucb2) and reproductive (kiss1, gpr54 and gnrh3) mRNAs. Furthermore, results showed that FD decreased HSI, and increased Lh levels and testicular preproghrelin and goat mRNAs, while sex steroids increased mid-brain nucb2, kiss1 and gpr54 mRNAs. Together, our results suggest that FD-induced inhibition of androgenesis resulted in diminished sperm quality associated with activation of the testicular ghrelinergic system, and negative feedback of 11-KT increased Lh level. The FD-induced testicular metabolic and hormonal system was impacted in goldfish exposed to sex steroids. However, the negative effects of FD on sperm quality were accelerated in E2-exposed goldfish due to estrogenic activity. This study provides novel information to better understand metabolic-associated reproductive disorders in fish.

• Klíčová slova
• Preproghrelin mRNA, nucleobindin2 mRNA, Hypothalamus-pituitary-testis, Sperm motility, Sperm production, Sperm velocity,
• MeSH
• estradiol * krev   farmakologie   MeSH
• karas zlatý * fyziologie   MeSH
• motilita spermií účinky léků   MeSH
• pohlavní steroidní hormony krev   metabolismus   MeSH
• potravinová deprivace * fyziologie   MeSH
• rozmnožování účinky léků   MeSH
• spermie účinky léků   fyziologie   MeSH
• testis účinky léků   metabolismus   MeSH
• testosteron * analogy a deriváty   krev   farmakologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 11-ketotestosterone MeSH Prohlížeč
• estradiol * MeSH
• pohlavní steroidní hormony MeSH
• testosteron * MeSH

Vinclozolin (VZ) is a pesticide that acts as an anti-androgen to impair reproduction in mammals. However, VZ-induced disruption of reproduction is largely unknown in fish. In the present study, we have established a combination exposure in which adult goldfish were exposed to VZ (30 and 100 μg/L), anti-androgen flutamide (Flu, 300 μg/L), and androgen testosterone (T, 1 μg/L) to better understand effects of VZ on reproductive endocrine system. mRNA levels of kisspeptin (kiss-1 and kiss-2) and its receptor (gpr54), salmon gonadotropin-releasing hormone (gnrh3) and androgen receptor (ar) in the mid-brain, and luteinizing hormone receptor (lhr) in the testis were analyzed and compared with those of control following 10 days of exposure. kiss-1 mRNA level was increased in goldfish exposed to 100 µg/L VZ and to Flu, while kiss-2 mRNA level was increased following exposure to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. gpr54 mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu and 100 µg/L VZ with T. gnrh3 mRNA level was increased in goldfish exposed to 100 µg/L VZ, to Flu, and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. The mid-brain ar mRNA level was increased in goldfish exposed to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. Testicular lhr mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu. These results suggest that VZ and Flu are capable of interfering with kisspeptin and GnRH systems to alter pituitary and testicular horonal functions in adult goldfish and the brain ar mediates VZ-induced disruption of androgen production.

• Klíčová slova
• Androgen receptor, Flutamide, Kisspeptin, Luteinizing hormone receptor, Salmon gonadotropin-releasing hormone, Testosterone,
• MeSH
• androgenní receptory genetika   MeSH
• androgeny farmakologie   MeSH
• antagonisté androgenů farmakologie   MeSH
• flutamid farmakologie   MeSH
• hormon uvolňující gonadotropiny genetika   MeSH
• karas zlatý genetika   MeSH
• kisspeptiny genetika   MeSH
• kyselina pyrrolidonkarboxylová analogy a deriváty   MeSH
• lékové interakce MeSH
• messenger RNA metabolismus   MeSH
• mozek účinky léků   metabolismus   MeSH
• oxazoly farmakologie   MeSH
• průmyslové fungicidy farmakologie   MeSH
• receptory LH genetika   MeSH
• receptory spřažené s G-proteiny genetika   MeSH
• rozmnožování účinky léků   MeSH
• rybí proteiny genetika   MeSH
• testis účinky léků   metabolismus   MeSH
• testosteron farmakologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• androgenní receptory MeSH
• androgeny MeSH
• antagonisté androgenů MeSH
• flutamid MeSH
• gonadotropin-releasing hormone-III MeSH Prohlížeč
• hormon uvolňující gonadotropiny MeSH
• kisspeptiny MeSH
• kyselina pyrrolidonkarboxylová MeSH
• messenger RNA MeSH
• oxazoly MeSH
• průmyslové fungicidy MeSH
• receptory LH MeSH
• receptory spřažené s G-proteiny MeSH
• rybí proteiny MeSH
• testosteron MeSH
• vinclozolin MeSH Prohlížeč