The objective of our in vitro study was to quantify the biochemical profile where the total polyphenol, flavonoid and phenolic acid content was determined. The antioxidant potential of microgreen extract from Trigonella foenum-graecum L., was measured molybdenum reducing power assay. Specifically, the study assessed parameters such as metabolic activity (AlamarBlueTM assay), membrane integrity (CFDA-AM assay), mitochondrial potential (JC-1 assay), as well as reactive oxygen species generation (NBT assay). In addition, the steroid hormone release in TM3 murine Leydig cells after 12 h and 24 h exposures were quantified by enzyme-linked immunosorbent assay. The gained results indicate the highest value in total flavonoid content (182.59+/-2.13 mg QE) determination, supported by a significant (108.25+/-1.27 mg TE) antioxidant activity. The effects on metabolic activity, cell membrane integrity, and mitochondrial membrane potential were found to be both time- and dose-dependent. Notably, a significant suppression in reactive oxygen species generation was confirmed at 150, 200 and 250 microg/ml after 24 h exposure. In addition, progesterone and testosterone release was stimulated up to 250 microg/ml dose of Trigonella, followed by a decline in both steroid production at 300 and 1000 microg/ml. Our results indicate, that Trigonella at lower experimental doses (up to 250 microg/ml) may positively affect majority of monitored cell parameters in TM3 Leydig cells. Overleaf, increasing experimental doses may negatively affect the intracellular parameters already after 12 h of in vitro exposure. Key words Microgreens, Trigonella foenum-graecum L., Fenugreek, Leydig cells, Male reproduction.
- MeSH
- antioxidancia * farmakologie izolace a purifikace MeSH
- Leydigovy buňky * účinky léků metabolismus MeSH
- membránový potenciál mitochondrií účinky léků MeSH
- myši MeSH
- reaktivní formy kyslíku metabolismus MeSH
- rostlinné extrakty * farmakologie izolace a purifikace MeSH
- testosteron metabolismus MeSH
- Trigonella * chemie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antioxidancia * MeSH
- reaktivní formy kyslíku MeSH
- rostlinné extrakty * MeSH
- testosteron MeSH
The central nervous system is a well-known steroidogenic tissue producing, among others, cholesterol metabolites such as neuroactive steroids, oxysterols and steroid hormones. It is well known that these endogenous molecules affect several receptor classes, including ionotropic GABAergic and NMDA glutamatergic receptors in neurons. It has been shown that also ionotropic purinergic (P2X) receptors are cholesterol metabolites' targets. Among P2X receptors, purinergic P2X4 and P2X7 receptors are expressed in microglia, the innate immune cells involved in the brain inflammatory response. In this study, we explore the ionotropic purinergic receptors modulation by cholesterol metabolites in microglia. Patch-clamp experiments were performed in BV2 cells, a murine microglia cell line, to evaluate effects of cholesterol metabolites using micro- and nanomolar concentrations. About P2X4 receptor, we found that testosterone butyrate (20 μM and 200 nM) and allopregnanolone (10 μM and 100 nM) both potentiated its current, while neither 25-hydroxycholesterol (10 μM and 100 nM) nor 17β-estradiol (1 μM) showed any effects. On the other hand, P2X7 receptor current was potentiated by allopregnanolone (10 μM) and 25-hydroxycholesterol (10 μM and 100 nM). Taken together, our data show that modulation of either P2X4 and P2X7 current is affected differently by cholesterol metabolites, suggesting a structure-activity relationship among these players. Identifying the possible link between purinergic transmission, microglia and cholesterol metabolites will allow to define new targets for drug development to treat neuroinflammation.
- Klíčová slova
- Microglia, Neuroactive steroids, Neuroinflammation, Oxysterols, P2X4 receptor, P2X7 receptor,
- MeSH
- buněčné linie MeSH
- mikroglie * metabolismus MeSH
- pregnanolon * metabolismus MeSH
- purinergní receptory P2X4 * metabolismus MeSH
- testosteron * metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- pregnanolon * MeSH
- purinergní receptory P2X4 * MeSH
- testosteron * MeSH
There is increasing pressure on meat producers worldwide due to the need for higher yields and improved meat quality. This is why anabolic androgenic steroids (AAS) have been widely used in most countries, due to their ability to accelerate animal muscle growth. However, out of concern for their side effects, EU states have banned their use and implemented control mechanisms. But they are reaching their limits, and therefore, it is necessary to look for new ways and investigate the mechanism of action of AAS on muscle tissue. This study replicated the administration of banned AAS (testosterone, nandrolone and their combination) and observed their effect on pig muscle. The pig model was purposely chosen for the study, as no such research has been carried out on this species. At the same time, pork is one of the most consumed meats in Europe. It focused on histological changes in muscle structure, specifically the size of muscle fibres and the number of satellite cells per muscle fibre. Furthermore, ultrastructural changes in muscle fibres, the diameter of myofibrils, the number of myofibrils per area, the distance between myofibrils and the size of sarcomeres were examined. The results using the techniques of histology, fluorescent labelling and transmission electron microscopy showed that, after the application of AAS, there is an increase in the diameter of muscle fibres, an increase in the diameter of myofibrils, a decrease in the number of myofibrils per surface area and, in the case of testosterone, an increase in the distance between myofibrils and an increase in the length of sarcomeres. There was also a significant increase in the number of satellite cells per muscle fibre. The detected statistically significant differences between control and experimental groups provide evidence that selected histological parameters could be additional mechanisms for detecting the presence of AAS in pork meat in the future.
- Klíčová slova
- anabolic steroids, electron microscopy, histology, muscles, pigs,
- MeSH
- anabolika * farmakologie MeSH
- androgeny farmakologie MeSH
- kosterní svalová vlákna * účinky léků ultrastruktura MeSH
- kosterní svaly účinky léků ultrastruktura MeSH
- myofibrily * účinky léků ultrastruktura MeSH
- nandrolon * farmakologie aplikace a dávkování MeSH
- prasata MeSH
- satelitní buňky kosterního svalu účinky léků MeSH
- testosteron * farmakologie aplikace a dávkování MeSH
- transmisní elektronová mikroskopie veterinární MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- anabolika * MeSH
- androgeny MeSH
- nandrolon * MeSH
- testosteron * MeSH
An important complication of prolonged support of the left ventricle with an assist device when implanted in patients with heart failure is unloading-induced cardiac atrophy. Our recent study suggested that sex-linked differences in the development of atrophy induced by heterotopic heart transplantation (HTX) do exist, however, the role of the environmental conditions dependent on plasma concentrations of sex hormones remains elusive. We aimed to compare the course of HTX-induced cardiac atrophy in male and female rats after gonadectomy with substitution of steroid hormones of the opposite sex. In a separate series of experiments, we evaluated the course of unloading-induced cardiac atrophy in the female heart transplanted into a male recipient and vice versa. Cardiac atrophy was assessed as the ratio of the transplanted heart weight to native heart weight (HW), which was determined 14 days after HTX. In female rats, studied in both experimental variants, HTx resulted in significantly smaller decreases in whole HW when compared to those observed in male rats exposed to the same experimental conditions (-9 ± 1 and - 11 + 1 vs. -44 ± 2 and -42 ± 2 %, p?0.05 in both cases). The dynamic of changes in left and right ventricle was similar as in the whole HW. Our results show that the process of unloading-induced cardiac atrophy exhibits important sex-linked differences and that attenuation of this process in female rats cannot be simply ascribed to the protective effects of estradiol or to the absence of deleterious actions of testosterone. Keywords: Cardiac atrophy, Sex differences, Gonadectomy, Hormonal substitution, Heterotopic heart transplantation, Mechanical heart unloading.
- MeSH
- atrofie * MeSH
- estradiol krev MeSH
- heterotopická transplantace * MeSH
- krysa rodu Rattus MeSH
- pohlavní dimorfismus * MeSH
- pohlavní steroidní hormony * krev MeSH
- potkani Wistar MeSH
- srdce MeSH
- testosteron krev MeSH
- transplantace srdce * škodlivé účinky MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- estradiol MeSH
- pohlavní steroidní hormony * MeSH
- testosteron MeSH
The purpose of this study was to determine the effects of resistance training (RT) alongside creatine-hydrochloride (Cr-HCl) or creatine monohydrate (CrM) supplementation on anabolic/catabolic hormones, strength, and body composition. Forty participants with an age range of 18-25 years were randomly divided into four groups (n=10): RT+Cr-HCl (0.03 g.kg-1 of body mass), RT+CrM-loading phase (CrM-LP) (0.3 g.kg-1 of body mass for five days (loading) and 0.03 g.kg-1 body mass for 51 days (maintenance)), RT+CrM-without loading phase (CrM-WLP) (0.03 g.kg-1 body mass), and RT+placebo (PL). The participants consumed supplements and performed RT with an intensity of 70-85 % 1RM for eight weeks. Before and after the training and supplementation period, strength (1RM), body composition (percent body fat (PBF), skeletal muscle mass (SMM), muscular cross-sectional area (MCSA)) and serum levels of testosterone, growth hormone (GH), insulin-like growth factor-1 (IGF-1), cortisol, adrenocorticotropic hormone (ACTH), follistatin and myostatin were measured. The results showed that in the supplementation groups, strength, arm and thigh MCSA, and SMM significantly increased, and PBF significantly decreased (P
- MeSH
- dospělí MeSH
- hormony * krev MeSH
- hydrokortison krev MeSH
- insulinu podobný růstový faktor I metabolismus MeSH
- kosterní svaly metabolismus účinky léků MeSH
- kreatin * farmakologie MeSH
- lidé MeSH
- lidský růstový hormon krev MeSH
- mladiství MeSH
- mladý dospělý MeSH
- odporový trénink * metody MeSH
- potravní doplňky * MeSH
- složení těla * účinky léků MeSH
- svalová síla * účinky léků MeSH
- testosteron krev MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- randomizované kontrolované studie MeSH
- Názvy látek
- hormony * MeSH
- hydrokortison MeSH
- insulinu podobný růstový faktor I MeSH
- kreatin * MeSH
- lidský růstový hormon MeSH
- testosteron MeSH
This study hypothesized that SCFA, acetate impacts positively on hypothalamic pyroptosis and its related abnormalities in experimentally induced PCOS rat model, possibly through NrF2/HIF1-α modulation. Eight-week-old female Wister rats were divided into groups (n = 5), namely control, PCOS, acetate and PCOS + acetate groups. Induction of PCOS was performed by administering 1 mg/kg body weight of letrozole for 21 days. After PCOS confirmation, the animals were treated with 200 mg/kg of acetate for 6 weeks. Rats with PCOS were characterized with insulin resistance, leptin resistance, increased plasma testosterone as well as degenerated ovarian follicles. There was also a significant increase in hypothalamic triglyceride level, triglyceride-glucose index, inflammatory biomarkers (SDF-1 and NF-kB) and caspase-6 as well as plasma LH and triglyceride. A decrease was observed in plasma adiponectin, GnRH, FSH, and hypothalamic GABA with severe inflammasome expression in PCOS rats. These were accompanied by decreased level of NrF2/HIF1-α, and the alterations were reversed when treated with acetate. Collectively, the present results suggest the therapeutic impact of acetate on hypothalamic pyroptosis and its related comorbidity in PCOS, a beneficial effect that is accompanied by modulation of NrF2/HIF1-α.
- Klíčová slova
- Acetate, GABA, Hypothalamus, Insulin resistance, PCOS, Pyroptosis,
- MeSH
- adiponektin metabolismus krev MeSH
- faktor 1 indukovatelný hypoxií - podjednotka alfa * metabolismus MeSH
- faktor 2 související s NF-E2 metabolismus MeSH
- folikuly stimulující hormon krev MeSH
- GABA metabolismus MeSH
- hormon uvolňující gonadotropiny metabolismus MeSH
- hypothalamus * metabolismus účinky léků patologie MeSH
- inzulinová rezistence MeSH
- krysa rodu Rattus MeSH
- leptin krev metabolismus MeSH
- letrozol farmakologie MeSH
- luteinizační hormon krev MeSH
- modely nemocí na zvířatech MeSH
- potkani Wistar * MeSH
- pyroptóza * účinky léků MeSH
- syndrom polycystických ovarií * chemicky indukované metabolismus farmakoterapie patologie MeSH
- testosteron krev MeSH
- triglyceridy krev metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- adiponektin MeSH
- faktor 1 indukovatelný hypoxií - podjednotka alfa * MeSH
- faktor 2 související s NF-E2 MeSH
- folikuly stimulující hormon MeSH
- GABA MeSH
- Hif1a protein, rat MeSH Prohlížeč
- hormon uvolňující gonadotropiny MeSH
- leptin MeSH
- letrozol MeSH
- luteinizační hormon MeSH
- Nfe2l2 protein, rat MeSH Prohlížeč
- testosteron MeSH
- triglyceridy MeSH
This study focused on continuous monitoring of the immunocontraceptive effect of Improvac® vaccine on the sexual activity of male goats determined by measuring plasma testosterone levels, testicular biometric and ejaculate examination. The animals in the experimental group (n=12) were administered two doses of 2 ml of Improvac® at a four-week interval; the animals in the control group (n=5) received 2 ml of saline. Blood collection, semen collection and testicular measurements were performed at 14-day intervals. A total of 8 samples were collected from each animal. In 9 animals a significant decrease (p<0.05) in testosterone concentration was observed two weeks after the first dose. At the end of the experiment (16 weeks), eight goats reached a testosterone concentration below the detection limit and one goat had a concentration of 0.47nmol/L. The testicular size was significantly (p<0.01) smaller four weeks after the first dose. At the end of the experiment, the testicular size was approximately three times smaller (p<0.001). Motility was 0% in two goats at the end of the experiment, 1% in one animal and 10% in one animal. The median sperm concentration was significantly lower (p<0.01) at the end of the study. A significant (p<0.0001) shift in the percentage of morphological changes was recorded eight weeks after the first administration. At the end of the study, there were five animals with azoospermia, two with 100% morphologically altered sperm, one with 99% and one with 96% morphologically altered sperm. In the three male goats, a significant skin reaction occurred after the first application, which resulted in an inadequate response to the treatment. Our results show that Improvac had a significant effect on the sexual function and sperm production in 9 out of 12 male goats.
- Klíčová slova
- Improvac, buck, immunocontraception, sperm,
- MeSH
- antikoncepce imunologická veterinární MeSH
- antikoncepční vakcíny farmakologie imunologie aplikace a dávkování MeSH
- kozy * fyziologie MeSH
- sexuální chování zvířat * účinky léků MeSH
- testis * účinky léků MeSH
- testosteron krev MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky veterinární MeSH
- Názvy látek
- antikoncepční vakcíny MeSH
- testosteron MeSH
There is a link between metabolism and reproduction as metabolic hormones affect hypothalamus-pituitary-testis (HPT) hormonal functions and vice versa. The aim of the present study was to investigate the effects of negative energy balance on the reproductive system in male goldfish exposed to testosterone (T) and 17β-estradiol (E2). Following 7 days of food deprivation (FD), ANOVA models showed significant FD × sex steroid interactions on sperm quality and circulating sex steroid levels. When FD effects were investigated, 11-ketotestosterone (11-KT) level and sperm motility and velocity decreased in food-deprived goldfish in the control group. In E2-exposed goldfish, FD decreased sperm production in addition to sperm motility and velocity that coincided with an elevation of circulating E2 level. However, FD did not significantly impact sex steroids and sperm quality in T-exposed goldfish. ANOVA models showed non-significant FD × sex steroid interactions for HSI, GSI, circulating luteinizing hormone (Lh) level, and metabolic (preproghrelin, goat and nucb2) and reproductive (kiss1, gpr54 and gnrh3) mRNAs. Furthermore, results showed that FD decreased HSI, and increased Lh levels and testicular preproghrelin and goat mRNAs, while sex steroids increased mid-brain nucb2, kiss1 and gpr54 mRNAs. Together, our results suggest that FD-induced inhibition of androgenesis resulted in diminished sperm quality associated with activation of the testicular ghrelinergic system, and negative feedback of 11-KT increased Lh level. The FD-induced testicular metabolic and hormonal system was impacted in goldfish exposed to sex steroids. However, the negative effects of FD on sperm quality were accelerated in E2-exposed goldfish due to estrogenic activity. This study provides novel information to better understand metabolic-associated reproductive disorders in fish.
- Klíčová slova
- Preproghrelin mRNA, nucleobindin2 mRNA, Hypothalamus-pituitary-testis, Sperm motility, Sperm production, Sperm velocity,
- MeSH
- estradiol * krev farmakologie MeSH
- karas zlatý * fyziologie MeSH
- motilita spermií účinky léků MeSH
- pohlavní steroidní hormony krev metabolismus MeSH
- potravinová deprivace * fyziologie MeSH
- rozmnožování účinky léků MeSH
- spermie účinky léků fyziologie MeSH
- testis účinky léků metabolismus MeSH
- testosteron * analogy a deriváty krev farmakologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 11-ketotestosterone MeSH Prohlížeč
- estradiol * MeSH
- pohlavní steroidní hormony MeSH
- testosteron * MeSH
This study aimed to investigate the effects of a single bench press (BP) vs. leg press (LP) resistance training sessions on testosterone, cortisol, C-reactive protein (CRP) interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) concentrations, and creatine kinase (CK) activity in strength-trained males. Eleven strength-trained males participated in a cross-over randomized trial, undergoing two experimental sessions each consisting of five sets of the BP or the LP exercise to volitional failure with a load corresponding to 50% of one-repetition maximum. Blood samples were taken at baseline (BA), immediately post (POST), and 1 h after the cessation of exercise (POST-1). A significant increase in IL-6 concentration from BA to POST-1 was observed during the LP condition (p = 0.004; effect size [ES] = 0.64). Additionally, a significant main effect of time was found for increasing testosterone concentrations from BA to POST exercise (p = 0.014; ES = 0.25). A significantly lower cortisol concentration at POST-1 compared to POST (p = 0.001; ES = 1.02) was noted in the BP condition. Furthermore, a significantly lower cortisol concentration was found at POST-1 in the BP compared to the LP condition (p = 0.022; ES = 1.3). A significant increase in CK activity was reported from BA to POST (p = 0.024; ES = 0.69) and POST-1 (p = 0.045; ES = 0.55) during the LP condition, and from BA to POST-1 (p = 0.014; ES = 0.96) during the BP condition. No significant differences were found in the CRP (p = 0.659) and TNF-α concentrations (p = 0.487). These results suggest that the amount of muscle mass engaged during the resistance exercise may influence the changes in IL-6 and cortisol concentrations. Larger muscle groups, as engaged in the LP, more likely lead to elevated concentrations of IL-6 myokine.
- Klíčová slova
- CRP, TNF-α, bench press, cortisol, creatine kinase, interleukin, myokine, testosterone,
- MeSH
- C-reaktivní protein metabolismus MeSH
- dospělí MeSH
- hydrokortison * krev MeSH
- interleukin-6 * krev MeSH
- klinické křížové studie MeSH
- kreatinkinasa krev MeSH
- lidé MeSH
- mladý dospělý MeSH
- odporový trénink * MeSH
- testosteron * krev MeSH
- TNF-alfa * krev MeSH
- zánět krev MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- randomizované kontrolované studie MeSH
- Názvy látek
- C-reaktivní protein MeSH
- hydrokortison * MeSH
- interleukin-6 * MeSH
- kreatinkinasa MeSH
- testosteron * MeSH
- TNF-alfa * MeSH
Perinatal testosterone, or its metabolite estradiol, organize the brain toward a male phenotype. Male rodents with insufficient testosterone during this period fail to display sexual behavior and partner preference for receptive females in adulthood. However, cohabitation with non-reproductive conspecifics under the influence of a D2 agonist facilitates the expression of conditioned partner preference via Pavlovian learning in gonadally intact male rats. In the present experiment, three groups of neonatal PD1 males (N = 12/group) were either gonadectomized (GDX), sham-GDX, or left intact and evaluated for social preferences and sexual behaviors as adults. We then examined whether the effects of GDX could be reversed by conditioning the males via cohabitation with receptive females under the effects of the D2 agonist quinpirole (QNP) or saline, along with the size of some brain regions, such as the sexually dimorphic nucleus of the preoptic area (SDN-POA), suprachiasmatic nucleus (SCN), posterior dorsal medial amygdala (MeApd) and ventromedial hypothalamus (VMH). Results indicated that neonatal GDX resulted in the elimination of male-typical sexual behavior, an increase in same-sex social preference, and a reduction of the area of the SDN-POA. However, GDX-QNP males that underwent exposure to receptive females in adulthood increased their social preference for females and recovered the size in the SDN-POA. Although neonatal GDX impairs sexual behavior and disrupts partner preference and brain dimorphism in adult male rats, Pavlovian conditioning under enhanced D2 agonism ameliorates the effects on social preference and restores brain dimorphism in the SDN-POA without testosterone.
- Klíčová slova
- Castration, Dopamine, Learning, Social preference, Testosterone,
- MeSH
- area praeoptica * metabolismus MeSH
- chinpyrol farmakologie MeSH
- kastrace MeSH
- krysa rodu Rattus MeSH
- mozek MeSH
- pohlavní dimorfismus * MeSH
- těhotenství MeSH
- testosteron farmakologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chinpyrol MeSH
- testosteron MeSH
