Thin films of binary C60/Ti composites, with various concentrations of Ti ranging from ~ 25% to ~ 70%, were deposited on microscopic glass coverslips and were tested for their potential use in bone tissue engineering as substrates for the adhesion and growth of bone cells. The novelty of this approach lies in the combination of Ti atoms (i.e., widely used biocompatible material for the construction of stomatological and orthopedic implants) with atoms of fullerene C60, which can act as very efficient radical scavengers. However, fullerenes and their derivatives are able to generate harmful reactive oxygen species and to have cytotoxic effects. In order to stabilize C60 molecules and to prevent their possible cytotoxic effects, deposition in the compact form of Ti/C60 composites (with various Ti concentrations) was chosen. The reactivity of C60/Ti composites may change in time due to the physicochemical changes of molecules in an air atmosphere. In this study, we therefore tested the dependence between the age of C60/Ti films (from one week to one year) and the adhesion, morphology, proliferation, viability, metabolic activity and potential DNA damage to human osteosarcoma cells (lines MG-63 and U-2 OS). After 7 days of cultivation, we did not observe any negative influence of fresh or aged C60/Ti layers on cell behavior, including the DNA damage response. The presence of Ti atoms resulted in improved properties of the C60 layers, which became more suitable for cell cultivation.

• MeSH
• Cell Adhesion drug effects   MeSH
• Time Factors MeSH
• Fullerenes chemistry   pharmacology   MeSH
• Humans MeSH
• Cell Line, Tumor MeSH
• Osteoblasts cytology   drug effects   metabolism   MeSH
• DNA Damage MeSH
• Cell Proliferation drug effects   MeSH
• Reactive Oxygen Species antagonists & inhibitors   metabolism   MeSH
• Titanium chemistry   pharmacology   MeSH
• Tissue Engineering MeSH
• Tissue Scaffolds * MeSH
• Cell Survival drug effects   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• fullerene C60 MeSH Browser
• Fullerenes MeSH
• Reactive Oxygen Species MeSH
• Titanium MeSH