Herein, we describe the preparation of liposomes with folate-targeting properties for the encapsulation of anti-sarcosine antibodies (antisarAbs@LIP) and sarcosine (sar@LIP). The competitive inhibitory effects of exogenously added folic acid supported the role of folate targeting in liposome internalization. We examined the effects of repeated administration on mice PC-3 xenografts. Sar@LIP treatment significantly increased tumor volume and weight compared to controls treated with empty liposomes. Moreover, antisarAbs@LIP administration exhibited a mild antitumor effect. We also identified differences in gene expression patterns post-treatment. Furthermore, Sar@LIP treatment resulted in decreased amounts of tumor zinc ions and total metallothioneins. Examination of the spatial distribution across the tumor sections revealed a sarcosine-related decline of the MT1X isoform within the marginal regions but an elevation after antisarAbs@LIP administration. Our exploratory results demonstrate the importance of sarcosine as an oncometabolite in PCa. Moreover, we have shown that sarcosine can be a potential target for anticancer strategies in management of PCa.

• MeSH
• Models, Biological MeSH
• Phosphatidylethanolamines MeSH
• Folic Acid metabolism   MeSH
• Humans MeSH
• Liposomes * chemistry   ultrastructure   MeSH
• Metallothionein metabolism   MeSH
• Disease Models, Animal MeSH
• Antibodies, Monoclonal administration & dosage   MeSH
• Mice MeSH
• Cell Line, Tumor MeSH
• Prostatic Neoplasms drug therapy   metabolism   pathology   MeSH
• Sarcosine antagonists & inhibitors   chemistry   MeSH
• Tumor Burden drug effects   MeSH
• Dose-Response Relationship, Drug MeSH
• Xenograft Model Antitumor Assays MeSH
• Zinc metabolism   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• dioleoyl phosphatidylethanolamine MeSH Browser
• Phosphatidylethanolamines MeSH
• Folic Acid MeSH
• Liposomes * MeSH
• Metallothionein MeSH
• Antibodies, Monoclonal MeSH
• Sarcosine MeSH
• Zinc MeSH