Protein modifications regulate both DNA repair levels and pathway choice. How each modification achieves regulatory effects and how different modifications collaborate with each other are important questions to be answered. Here, we show that sumoylation regulates double-strand break repair partly by modifying the end resection factor Sae2. This modification is conserved from yeast to humans, and is induced by DNA damage. We mapped the sumoylation site of Sae2 to a single lysine in its self-association domain. Abolishing Sae2 sumoylation by mutating this lysine to arginine impaired Sae2 function in the processing and repair of multiple types of DNA breaks. We found that Sae2 sumoylation occurs independently of its phosphorylation, and the two modifications act in synergy to increase soluble forms of Sae2. We also provide evidence that sumoylation of the Sae2-binding nuclease, the Mre11-Rad50-Xrs2 complex, further increases end resection. These findings reveal a novel role for sumoylation in DNA repair by regulating the solubility of an end resection factor. They also show that collaboration between different modifications and among multiple substrates leads to a stronger biological effect.

• MeSH
• DNA vazebné proteiny genetika   MeSH
• dvouřetězcové zlomy DNA MeSH
• endodeoxyribonukleasy genetika   MeSH
• endonukleasy genetika   MeSH
• exodeoxyribonukleasy genetika   MeSH
• fosforylace MeSH
• lidé MeSH
• oprava DNA spojením konců genetika   MeSH
• oprava DNA genetika   MeSH
• poškození DNA genetika   MeSH
• rozpustnost MeSH
• Saccharomyces cerevisiae - proteiny genetika   MeSH
• Saccharomyces cerevisiae MeSH
• sumoylace genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• DNA vazebné proteiny MeSH
• endodeoxyribonukleasy MeSH
• endonukleasy MeSH
• exodeoxyribonukleasy MeSH
• MRE11 protein, S cerevisiae MeSH Prohlížeč
• RAD50 protein, S cerevisiae MeSH Prohlížeč
• Saccharomyces cerevisiae - proteiny MeSH
• SAE2 protein, S cerevisiae MeSH Prohlížeč
• XRS2 protein, S cerevisiae MeSH Prohlížeč