The current evidence of regulatory effect of smoke-water (SW) and karrikinolide (KAR(1)) on the concentrations of endogenous cytokinins in plants partly explain the basis for their growth stimulatory activity. Karrikinolide (KAR1) which is derived from smoke-water (SW) is involved in some physiological aspects in the life-cycle of plants. This suggests a potential influence on the endogenous pool (quantity and quality) of phytohormones such as cytokinins (CKs). In the current study, the effect of SW (1:500; 1:1000; 1:1500 v/v dilutions) and KAR1 (10(-7); 10(-8); 10(-9) M) applied during micropropagation of Eucomis autumnalis subspecies autumnalis on the ex vitro growth and CKs after 4 months post-flask duration was evaluated. The interactions of SW and KAR(1) with benzyladenine (BA), α-naphthaleneacetic acid (NAA) or BA+NAA were also assessed. Plants treated with SW (1:500) and KAR1 (10(-8) M) demonstrated superior growth in terms of the rooting, leaf and bulb sizes and fresh biomass than the control and plants treated with BA and BA+NAA. However, plant growth was generally inhibited with either SW (1:500) or KAR1 (10(-8) M) and BA when compared to BA (alone) treatment. Relative to NAA treatment, the presence of KAR(1) (10(-7) M) with NAA significantly increased the leaf area and fresh biomass. Both SW and KAR1-treated plants accumulated more total CKs, mainly isoprenoid-type than the control and NAA-treated plants. The highest CK content was also accumulated in SW (1:500) with BA+NAA treatments. Similar stimulatory effects were observed with increasing concentrations of KAR(1) and BA. The current findings establish that SW and KAR1 exert significant influence on the endogenous CK pools. However, the better growth of plants treated with SW and KAR1 treatments was not exclusively related to the endogenous CKs.

• Klíčová slova
• Acclimatization, Asparagaceae, Conservation, Medicinal plants, Plant growth regulators, UHPLC,
• MeSH
• aklimatizace * MeSH
• Asparagaceae účinky léků   růst a vývoj   fyziologie   MeSH
• biomasa MeSH
• cytokininy analýza   metabolismus   MeSH
• furany farmakologie   MeSH
• kořeny rostlin účinky léků   růst a vývoj   fyziologie   MeSH
• kouř MeSH
• kyseliny naftalenoctové metabolismus   MeSH
• léčivé rostliny MeSH
• lipnicovité MeSH
• listy rostlin účinky léků   růst a vývoj   fyziologie   MeSH
• pyrany farmakologie   MeSH
• regulátory růstu rostlin analýza   metabolismus   MeSH
• voda chemie   MeSH
• výhonky rostlin účinky léků   růst a vývoj   fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 1-naphthaleneacetic acid MeSH Prohlížeč
• cytokininy MeSH
• furany MeSH
• karrikinolide MeSH Prohlížeč
• kouř MeSH
• kyseliny naftalenoctové MeSH
• pyrany MeSH
• regulátory růstu rostlin MeSH
• voda MeSH