Calcofluor-allied optical brightener Rylux BSU stimulated spore germination rate in Trichophyton mentagrophytes and Aspergillus fumigatus both if supplemented into Sabouraud glucose agar and if used for pretreatment of spore suspension prior to inoculation at low concentrations. Maximum stimulation of germination was obtained if 0.2% Rylux BSU was used for pretreatment in aqueous solution for 1 d prior to inoculation (130% in T. mentagrophytes and 150% in A. fumigatus, respectively). Pretreatment with 1% Rylux BSU provided strong protection against UV-irradiation and resulted in increased yields of cultural variants after UV-irradiation.

• MeSH
• Aspergillus fumigatus drug effects   growth & development   metabolism   radiation effects   MeSH
• Benzenesulfonates pharmacology   MeSH
• Fluorescent Dyes MeSH
• Culture Media MeSH
• Microscopy, Electron, Scanning MeSH
• Spores, Fungal growth & development   MeSH
• Trichophyton drug effects   growth & development   metabolism   radiation effects   MeSH
• Ultraviolet Rays MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Benzenesulfonates MeSH
• Fluorescent Dyes MeSH
• Culture Media MeSH
• Rylux BSU MeSH Browser

The fluorescence brightener Rylux BSU (RBSU) showed an affinity for polysaccharide components of cell walls and accumulated in the extension zones of hyphal apices in Basidiobolus ranarum. It inhibited the polarized growth of mycelial hyphae and induced isotropic growth resulting in spherical thick-walled cells up to 456 microm in diameter. On the inner cell wall surface, massive protuberances were formed. The cell wall and protuberances were positive in PAS and the Grocott method and stained with fluorochromes Blankophor BA, Calcofluor, Uvitex 2B, Rylux BSU and FITC-labeled WGA- and ConA-lectins. The WGA-FITC fluorescence intensity of the wall's outermost layer, if not connected with neighbouring cells, and the fluorescence intensity of the innermost layer and of some protuberances mainly in their apical parts were on the average twice higher than the fluorescence intensity of the remaining wall material. RBSU binding to the cell wall material was stable. The process of converting from polarized to isotropic growth was reversible, depending upon contact with RBSU-containing medium. Repeated transfers of cells from RBSU-containing medium to an RBSU-free medium resulted in the development of apical swollen dumbbell-shaped cells.

• MeSH
• Benzenesulfonates pharmacology   MeSH
• Fluorescent Dyes pharmacology   MeSH
• Fungi cytology   drug effects   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Benzenesulfonates MeSH
• Fluorescent Dyes MeSH
• Rylux BSU MeSH Browser

Spore walls of Backusella lamprospora (Mucorales) were stained with ten fluorescent brighteners (FB) and the intensity of their fluorescence was determined. The fluorescence was most intense with Uvitex 2B (100%), other brighteners yielding lower fluorescence intensities: Blankophor BA 267% and BA 200% about 75%, Rylux BSU about 50%, other Rylux agents 10-30%. The agents most suitable for microscopic diagnostics of human and animal mycoses are Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU, and also Rylux BS and PRS. The regulation of excessive fluorescence of fungal cells during microscopic observation is discussed. For the purposes of microscopic diagnosis of human and animal mycosis Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU and, possibly, Rylux BS and PRS are recommended.

• MeSH
• Staining and Labeling MeSH
• Cell Wall chemistry   MeSH
• Fluorescent Dyes * MeSH
• Humans MeSH
• Mucorales isolation & purification   MeSH
• Mycoses diagnosis   MeSH
• Flow Cytometry * MeSH
• Spores, Fungal isolation & purification   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH
• Names of Substances
• Fluorescent Dyes * MeSH