Owing to their high surface area, stability, and functional groups on the surface, iron oxide hydroxide nanoparticles have attracted attention as enzymatic support. In this work, a chemometric approach was performed, aiming at the optimization of the horseradish peroxidase (HRP) immobilization process on Δ-FeOOH nanoparticles (NPs). The enzyme/NPs ratio (X1), pH (X2), temperature (X3), and time (X4) were the independent variables analyzed, and immobilized enzyme activity was the response variable (Y). The effects of the factors were studied using a factorial design at two levels (-1 and 1). The biocatalyst obtained was evaluated for the ferulic acid (FA) removal, a pollutant model. The materials were characterized by X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The SEM images indicated changes in material morphology. The independent variables X1 (-0.57), X2 (0.71), and X4 (0.42) presented the significance effects estimate. The variable combinations resulted in two significance effects estimates, X1*X2 (-0.57) and X2*X4 (0.39). The immobilized HRP by optimized conditions (X1 = 1/63 (enzyme/NPs ratio, X2 = pH 8, X4 = 60 °C, and 30 min) showed high efficiency for FA oxidation (82%).

• Klíčová slova
• bioremediation, horseradish peroxidase, immobilization, iron oxide hydroxide,
• MeSH
• biokatalýza MeSH
• difrakce rentgenového záření MeSH
• enzymy imobilizované metabolismus   MeSH
• křenová peroxidasa metabolismus   ultrastruktura   MeSH
• kyseliny kumarové chemie   metabolismus   MeSH
• nanočástice chemie   MeSH
• oxidace-redukce MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• železité sloučeniny chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• enzymy imobilizované MeSH
• ferric oxyhydroxide MeSH Prohlížeč
• ferulic acid MeSH Prohlížeč
• křenová peroxidasa MeSH
• kyseliny kumarové MeSH
• železité sloučeniny MeSH