Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.

• Klíčová slova
• Ribes, closterovirus, currant, recombinants/recombination,
• MeSH
• Closterovirus klasifikace   genetika   izolace a purifikace   ultrastruktura   MeSH
• fylogeneze MeSH
• genetická variace MeSH
• genom virový MeSH
• genomika metody   MeSH
• nemoci rostlin virologie   MeSH
• otevřené čtecí rámce MeSH
• rekombinace genetická MeSH
• Ribes virologie   MeSH
• RNA virová MeSH
• sekvence aminokyselin MeSH
• vysoce účinné nukleotidové sekvenování MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH
• Názvy látek
• RNA virová MeSH