Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.

• Klíčová slova
• Ribes, closterovirus, currant, recombinants/recombination,
• MeSH
• Closterovirus klasifikace   genetika   izolace a purifikace   ultrastruktura   MeSH
• fylogeneze MeSH
• genetická variace MeSH
• genom virový MeSH
• genomika metody   MeSH
• nemoci rostlin virologie   MeSH
• otevřené čtecí rámce MeSH
• rekombinace genetická MeSH
• Ribes virologie   MeSH
• RNA virová MeSH
• sekvence aminokyselin MeSH
• vysoce účinné nukleotidové sekvenování MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH
• Názvy látek
• RNA virová MeSH

Two sensitive methods, RT-PCR with phenol-extracted RNA or Triton X-100-released RNA and immunocapture RT-PCR (IR-RT-PCR) were used for the detection of Beet yellows virus (BYV) in young and old leaves of Tetragonia expansa and sugar beet (Beta vulgaris) and in sugar beet roots. Four oligonucleotide primer pairs proved suitable for the detection of BYV. The release of BYV RNA with Triton X-100 was shown to be a very effective and easy as compared to isolation of total RNA by phenol extraction with the same or higher sensitivity of subsequent PCR. Using the Triton X-100 release of RNA and IC-RT-PCR the sensitivity of detection was so high that pg amounts of BYV RNA occurring in dilutions up to 10(-6) of saps from young Tetragonia and sugar beet leaves could be detected.

• MeSH
• Aizoaceae virologie   MeSH
• Beta vulgaris virologie   MeSH
• Closterovirus genetika   izolace a purifikace   MeSH
• DNA primery MeSH
• fenol MeSH
• kořeny rostlin virologie   MeSH
• listy rostlin virologie   MeSH
• oktoxynol MeSH
• polymerázová řetězová reakce s reverzní transkripcí * metody   MeSH
• RNA virová analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA primery MeSH
• fenol MeSH
• oktoxynol MeSH
• RNA virová MeSH