An impedimetric lectin biosensor for the detection of changes in the glycan structure of antibodies isolated from human serum is here correlated with the progression of rheumatoid arthritis (RA). The biosensor was built up from a mixed self-assembled monolayer (SAM) on gold consisting of two different thiolated zwitterionic derivatives, carboxybetaine and sulfobetaine, to resist nonspecific interactions. The carboxyl-terminated one was applied also for the covalent immobilization of lectin Ricinus communis agglutinin I (RCA-I). The process of building a bioreceptive layer was optimized and characterized using a diverse range of techniques. Impedimetric assays were integrated on a chip consisting of eight gold working electrodes, which is an important step toward the achievement of a moderate level of multiplexing for the analysis of human serum samples. At the end, the results obtained by the impedimetric analysis of immunoglobulins G (IgGs) isolated from serum samples were compared with those of two other standard bioanalytical methods employing lectins, that is, lectin microarrays (MAs) and enzyme-linked lectin binding assays (ELLBAs). The impedimetric results agreed very well with the DAS28 index (RA disease activity score 28), suggesting that impedimetric assays could be used for the development of a new diagnostic procedure sensitive to glycosylation changes in human IgGs and thus RA progression.

• MeSH
• biosenzitivní techniky * přístrojové vybavení   metody   MeSH
• čipová analýza proteinů * přístrojové vybavení   metody   MeSH
• elektrody MeSH
• glykosylace MeSH
• imunoanalýza přístrojové vybavení   metody   MeSH
• imunoglobulin G analýza   krev   MeSH
• lidé MeSH
• polysacharidy analýza   krev   MeSH
• revmatoidní artritida krev   MeSH
• rostlinné lektiny chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• imunoglobulin G MeSH
• polysacharidy MeSH
• Ricinus communis agglutinin-1 MeSH Prohlížeč
• rostlinné lektiny MeSH