Nejvíce citovaný článek - PubMed ID 27016082
A method to decompose spectral changes in Synechocystis PCC 6803 during light-induced state transitions
The slow kinetic phases of the chlorophyll a fluorescence transient (induction) are valuable tools in studying dynamic regulation of light harvesting, light energy distribution between photosystems, and heat dissipation in photosynthetic organisms. However, the origin of these phases are not yet fully understood. This is especially true in the case of prokaryotic oxygenic photoautotrophs, the cyanobacteria. To understand the origin of the slowest (tens of minutes) kinetic phase, the M-T fluorescence decline, in the context of light acclimation of these globally important microorganisms, we have compared spectrally resolved fluorescence induction data from the wild type Synechocystis sp. PCC 6803 cells, using orange (λ = 593 nm) actinic light, with those of mutants, ΔapcD and ΔOCP, that are unable to perform either state transition or fluorescence quenching by orange carotenoid protein (OCP), respectively. Our results suggest a multiple origin of the M-T decline and reveal a complex interplay of various known regulatory processes in maintaining the redox homeostasis of a cyanobacterial cell. In addition, they lead us to suggest that a new type of regulatory process, operating on the timescale of minutes to hours, is involved in dissipating excess light energy in cyanobacteria.
- Klíčová slova
- Fluorescence quenching, Interplay of regulatory processes, Kautsky effect, Photoprotection, Synechocystis, The M–T phase,
- MeSH
- bakteriální proteiny genetika metabolismus MeSH
- chlorofyl a MeSH
- chlorofyl chemie genetika metabolismus MeSH
- diuron chemie MeSH
- fluorescence MeSH
- fluorescenční spektrometrie MeSH
- fykobilizomy genetika metabolismus MeSH
- kyanid draselný chemie MeSH
- luminiscenční měření MeSH
- světlo MeSH
- Synechocystis chemie genetika metabolismus MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální proteiny MeSH
- chlorofyl a MeSH
- chlorofyl MeSH
- diuron MeSH
- fykobilizomy MeSH
- kyanid draselný MeSH
- orange carotenoid protein, Synechocystis MeSH Prohlížeč
