IgA nephropathy is diagnosed by renal biopsy, an invasive procedure with a risk of significant complications. Noninvasive approaches are needed for possible diagnostic purposes and especially for monitoring disease activity or responses to treatment. In this pilot project, we assessed the utility of urine samples as source of biomarkers of IgA nephropathy. We used spot urine specimens from 19 healthy controls, 11 patients with IgA nephropathy, and 8 renal-disease controls collected on day of renal biopsy. Urine samples were analyzed using untargeted metabolomic and targeted proteomic analyses by several experimental techniques: liquid chromatography coupled with mass spectrometry, immunomagnetic isolation of target proteins coupled with quantitation by mass spectrometry, and protein arrays. No single individual biomarker completely differentiated the three groups. Therefore, we tested the utility of several markers combined in a panel. Discriminant analysis revealed that combination of seven markers, three metabolites (dodecanal, 8-hydroxyguanosine, and leukotriene C4), three proteins (α1-antitrypsin, IgA-uromodulin complex, and galactose-deficient IgA1), and heparan sulfate, differentiated patients with IgA nephropathy from patients with other renal diseases and healthy controls. Future studies are needed to validate these preliminary findings and to determine the power of these urinary markers for assessment of responses to therapy.

• MeSH
• aldehydy moč   MeSH
• alfa-1-antitrypsin moč   MeSH
• biologické markery moč   MeSH
• dospělí MeSH
• guanosin analogy a deriváty   moč   MeSH
• heparitinsulfát moč   MeSH
• IgA nefropatie patologie   moč   MeSH
• imunoglobulin A moč   MeSH
• leukotrien C4 moč   MeSH
• lidé středního věku MeSH
• lidé MeSH
• metabolom * MeSH
• proteom * MeSH
• senioři MeSH
• studie případů a kontrol MeSH
• uromodulin moč   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 8-hydroxyguanosine MeSH Prohlížeč
• aldehydy MeSH
• alfa-1-antitrypsin MeSH
• biologické markery MeSH
• guanosin MeSH
• heparitinsulfát MeSH
• imunoglobulin A MeSH
• leukotrien C4 MeSH
• proteom * MeSH
• uromodulin MeSH