Non-random gene organization in eukaryotes plays a significant role in genome evolution. Here, we investigate the origin of a biosynthetic gene cluster for production of defence compounds in oat-the avenacin cluster. We elucidate the structure and organisation of this 12-gene cluster, characterise the last two missing pathway steps, and reconstitute the entire pathway in tobacco by transient expression. We show that the cluster has formed de novo since the divergence of oats in a subtelomeric region of the genome that lacks homology with other grasses, and that gene order is approximately colinear with the biosynthetic pathway. We speculate that the positioning of the late pathway genes furthest away from the telomere may mitigate against a 'self-poisoning' scenario in which toxic intermediates accumulate as a result of telomeric gene deletions. Our investigations reveal a striking example of adaptive evolution underpinned by remarkable genome plasticity.

• MeSH
• hybridizace in situ fluorescenční MeSH
• jedlá semena genetika   MeSH
• metabolické sítě a dráhy genetika   MeSH
• molekulární evoluce MeSH
• multigenová rodina MeSH
• odolnost vůči nemocem genetika   MeSH
• oves genetika   metabolismus   MeSH
• repetitivní sekvence nukleových kyselin MeSH
• saponiny biosyntéza   chemie   genetika   MeSH
• sekvenování celého genomu MeSH
• sekvenování transkriptomu MeSH
• syntenie genetika   MeSH
• tabák metabolismus   MeSH
• telomery genetika   MeSH
• vysoce účinné nukleotidové sekvenování MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• avenacin A 1 MeSH Prohlížeč
• saponiny MeSH