Influence of in vitro temperature on sperm antioxidant enzyme activity, thiobarbituric acid-reactive substance (TBARS) content and motility parameters was evaluated in sterlet Acipenser ruthenus and rainbow trout Oncorhynchus mykiss. Sperm activation was conducted at 4, 14 and 24 °C in both species. Duration of motility was significantly longer at 4 °C than at 14 and 24 °C in both species. At 60 s post-activation, the velocity of sterlet spermatozoa was highest at 24 °C. This trend continued to 420 s post-activation. In rainbow trout, at 10 s post-activation, the highest velocity was observed at 14 °C. Significantly higher catalase activity was seen at 4 °C in both species. No significant difference in spermatozoon superoxide dismutase activity among temperatures was observed. In sterlet, TBARS content was significantly higher at 24 °C compared to other temperatures, but, in rainbow trout, it was highest at 4 °C. The results presume species-specific level of antioxidant enzyme activity and TBARS content at studied temperatures.

• Keywords
• Antioxidant enzyme, Fish, Sperm function, Temperature,
• MeSH
• Antioxidants metabolism   MeSH
• Thiobarbituric Acid Reactive Substances metabolism   MeSH
• Sperm Motility * MeSH
• Oncorhynchus mykiss physiology   MeSH
• Lipid Peroxidation MeSH
• Fishes physiology   MeSH
• Spermatozoa enzymology   MeSH
• Superoxide Dismutase metabolism   MeSH
• Temperature * MeSH
• Animals MeSH
• Check Tag
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Antioxidants MeSH
• Thiobarbituric Acid Reactive Substances MeSH
• Superoxide Dismutase MeSH