The tear film at the ocular surface is covered by a thin layer of lipids. This oily phase stabilizes the film by decreasing its surface tension and improving its viscoelastic properties. Clinically, destabilization and rupture of the tear film are related to dry eye disease and are accompanied by changes in the quality and quantity of tear film lipids. In dry eye, eye drops containing oil-in-water emulsions are used for the supplementation of lipids and surface-active components to the tear film. We explore in detail the biophysical aspects of interactions of specific surface-active compounds, cetalkonium chloride and poloxamer 188, which are present in oil-in-water emulsions, with tear lipids. The aim is to better understand the macroscopically observed eye drops-tear film interactions by rationalizing them at the molecular level. To this end, we employ a multi-scale approach combining experiments on human meibomian lipid extracts, measurements using synthetic lipid films, and in silico molecular dynamics simulations. By combining these methods, we demonstrate that the studied compounds specifically interact with the tear lipid film enhancing its structure, surfactant properties, and elasticity. The observed effects are cooperative and can be further modulated by material packing at the tear-air interface.

• Klíčová slova
• Langmuir trough, dilatation rheology, fluorescence microscopy, meibum, molecular dynamics, tear film, tear film lipid layer,
• MeSH
• fluorescenční mikroskopie * metody   MeSH
• kvartérní amoniové sloučeniny chemie   MeSH
• lidé MeSH
• lipidy * chemie   MeSH
• mastné alkoholy chemie   MeSH
• meibomské žlázky metabolismus   MeSH
• poloxamer chemie   MeSH
• simulace molekulární dynamiky * MeSH
• slzy * chemie   MeSH
• teoretické modely MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cetalkonium chloride MeSH Prohlížeč
• kvartérní amoniové sloučeniny MeSH
• lipidy * MeSH
• mastné alkoholy MeSH
• poloxamer MeSH