Levels of phenotypic and genotypic diversity among seven Megasphaera elsdenii strains recovered from rumen contents of cattle, sheep and lambs were determined by a combination of antibiotic-resistance analysis and PCR fingerprint techniques targeted both to the ribosomal RNA operon (ARDRA, RISA) and the whole genome (ERIC-PCR, RAPD-PCR). Despite exhibiting different antibiotic resistance profiles, the tested strains represent genetically nearly identical isolates. Close genetic relatedness was found among M. elsdenii isolates that originated from vastly different habitats worldwide, as revealed by the comparison of 16S rDNA sequences.

• MeSH
• Rumen microbiology   MeSH
• DNA, Bacterial genetics   MeSH
• DNA Fingerprinting MeSH
• Phenotype MeSH
• Phylogeny MeSH
• Genetic Variation * MeSH
• Genotype MeSH
• Megasphaera classification   genetics   MeSH
• Microbial Sensitivity Tests methods   MeSH
• Molecular Sequence Data MeSH
• Sheep MeSH
• RNA, Ribosomal, 16S classification   MeSH
• Cattle MeSH
• Animals MeSH
• Check Tag
• Cattle MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• DNA, Bacterial MeSH
• RNA, Ribosomal, 16S MeSH

The dual-substrate utilization pattern in cultures of five ruminal strains of Butyrivibrio fibrisolvens growing on glucose and xylose was investigated. Strains ATCC 19171 and 86 utilized glucose and xylose simultaneously. Other strains exhibited diauxic growth. Strains X1 and CE 51 exhibited classical diauxic growth in which glucose was utilized during the first phase. Strain X2D62 displayed atypical diauxic growth in which slow utilization of xylose was followed by rapid utilization of glucose after the xylose depletion. The ATP-dependent phosphorylation of glucose was found in all strains tested. The phosphoenolpyruvate-dependent phosphorylation of glucose was detected only in B. fibrisolvens CE 51.

• Publication type
• Journal Article MeSH

A rumen strain of Megasphaera elsdenii was grown on glucose and lactate in monensin-free and monensin-supplemented medium (10 mg/L). Monensin had no effect on growth rate, growth yields, metabolic pattern and composition of cells. Growth yields of dry matter and protein were higher in cultures supplied with glucose than in cultures supplied with lactate. The bacterium compensated the lower gain of energy from fermentation of lactate by rapid utilization of this substrate. Cells grown on glucose contained more saccharide and less protein than lactate-grown cells.

• MeSH
• Rumen microbiology   MeSH
• Glucose metabolism   MeSH
• Lactose metabolism   MeSH
• Monensin pharmacology   MeSH
• Veillonellaceae drug effects   growth & development   metabolism   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Glucose MeSH
• Lactose MeSH
• Monensin MeSH