Most cited article - PubMed ID 27716773
Enhanced Mitogenic Activity of Recombinant Human Vascular Endothelial Growth Factor VEGF121 Expressed in E. coli Origami B (DE3) with Molecular Chaperones
Vascular endothelial growth factor-A165 (VEGF-A165) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A165 and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A165 stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A165 on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A165 have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering.
- Keywords
- adult stem cells, basic fibroblast growth factor (bFGF), cell adhesion, cell proliferation, endothelial cells, heterologous expression, recombinant vascular endothelial growth factor (VEGF), regenerative medicine, tissue engineering, vascular replacements,
- MeSH
- Cell Adhesion drug effects MeSH
- Human Umbilical Vein Endothelial Cells cytology metabolism MeSH
- Fibroblast Growth Factor 2 chemistry genetics pharmacology MeSH
- Stem Cells cytology metabolism MeSH
- Humans MeSH
- Swine MeSH
- Cell Proliferation drug effects MeSH
- Recombinant Proteins chemistry pharmacology MeSH
- Vascular Endothelial Growth Factor A chemistry genetics pharmacology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fibroblast Growth Factor 2 MeSH
- Recombinant Proteins MeSH
- Vascular Endothelial Growth Factor A MeSH
- VEGFA protein, human MeSH Browser
