The aim of this study was to estimate genetic parameters for the reproductive traits of boars based on single-nucleotide polymorphism data. A total of 109,836 semen samples from 2249 boars were collected between 2010 and 2022. Five basic traits were assessed: sperm volume, sperm concentration, motility, number of abnormal sperm, and, for the first time for the local population, libido. In addition, two derived traits were assessed: total sperm count and number of functional sperm. Genetic parameters were estimated using the single-step genomic best linear unbiased prediction method (ssGBLUP). Dam and sire breeds were evaluated separately. The five basic traits were evaluated using five-trait models, while the two derived traits were evaluated using single-trait models. The heritability coefficients had lower values for all sperm quality traits with both methods. For the basic traits, the heritability ranged from 0.099 to 0.342. The greatest difference between dam and sire breeds was observed for the heritability of the sperm concentration trait (0.099 and 0.271, respectively). The heritability of the libido trait was twice as high for boars of sire breeds as it was for boars of dam breeds. The genetic parameters estimated with ssGBLUP can be used in routine genetic evaluations to improve the pig breeding process.

• Klíčová slova
• heritability, libido, pig, sperm characteristics, ssGBLUP,
• MeSH
• fenotyp MeSH
• genomika MeSH
• počet spermií MeSH
• prasata genetika   MeSH
• sperma * MeSH
• spermie * MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• komentáře MeSH
• práce podpořená grantem MeSH

Selenium is an essential compound which can influence the fertility of boars by a greater margin. In past decades, research was mainly focused on a bioavailability of various selenium forms and the effect on semen quality. Recently, nanotechnology has expanded the possibilities of selenium supplementation research. Twenty-one Duroc boars (three groups with seven boars each) were included in this experiment with the first group being a control group with no selenium supplementation, and the second group being supplemented with 0.3 mg Se/kg of selenium in inorganic form of Na2SeO3. The third group was supplemented with selenium nanoparticles (100 nm) at the same dose as that of the second group. The experiment lasted for 126 days (three spermatogenesis cycles of boars) and the antioxidant parameters of boar semen were analysed at 42, 84 and 126 days, respectively. The antioxidant parameters (DPPH, FRAP, DMPD, GSH, GSSG) were not influenced by both Se2NO3 and selenium nanoparticle supplementation during this experiment. At the end of the monitored period, significantly higher (p < 0.004) antioxidant readings were observed by using the ABTS method but not the DPPH, DMPD and FRAP methods on the supplemented groups compared to the control. Moreover, selenium-nanoparticle-supplemented groups showed elevated glutathione peroxidase activity in the seminal fluid (p < 0.008). However, the selenium nanoparticle supplementation has not shown an improving effect on sperm quality. This could be considered as a safe alternative to inorganic selenium as well as having a potential to enhance the antioxidant properties of the semen of boars.

• Klíčová slova
• antioxidants, boars, nanoparticles, selenium, semen,
• Publikační typ
• časopisecké články MeSH

This study analysed the effect of sow parity (P), terminal boars (TB), and their combination on reproductive parameters of sows, losses of piglets, and their individual live-weight (LW) and average daily gain (ADG) from birth to weaning. A total of 120 sows of Large White × Landrace hybrid combination from the first to the fourth parity (30 sows per parity) were included in the observation. The sows were inseminated by terminal boars of Pietrain (Pn), Large Whitesireline (LWSL), and Duroc (D) breeds and Duroc × Large Whitesirelinie (D × LWSL), Large Whitesirelinie × Pietrain (LWSL × Pn), and Duroc × Pietrain (D × Pn) hybrid combinations (20 sows per terminal boar population). The results proved a significant effect of P on the total numbers of piglets (TN), the numbers of stillborn piglets (SB), and the pre-weaning losses of piglets (L) (p ≤ 0.01) with the lowest losses found in the third parity sows. A significant effect of TB was confirmed for TN, the numbers of live-born piglets (LB), SB, the numbers of mummified piglets (M) and non-viable piglets (N), and (L) (p ≤ 0.01). The sows inseminated by D and LWSL boars showed the lowest total numbers of piglets but also the highest survivability of piglets until weaning. Significant effects of interaction between P and TB were observed for the TN, SB, N, and L (p ≤ 0.05). Evaluation of growth parameters in piglets (n = 1547) showed that P significantly influenced LW in the first three weeks of life (p ≤ 0.01) and ADG from birth to Day 7 and from Day 15 to Day 21 (p ≤ 0.01). Additionally, a significant effect (p ≤ 0.01) of TB was proven on individual weight and ADG in all the observed time intervals, with the highest growth found in the piglets of the D and LWSL boars. Significant interactions (p ≤ 0.01) between P and TB were found for LW and ADG in all the time intervals.

• Klíčová slova
• average daily gain, live-weight, losses of piglets, parity of sows, terminal boars,
• Publikační typ
• časopisecké články MeSH

Hydrogen sulphide (H2S) is involved in the physiology and pathophysiology of different cell types, but little is known about its role in sperm cells. Because of its reducing properties, we hypothesise that H2S protects spermatozoa against the deleterious effects of oxidative stress, a condition that is common to several male fertility disorders. This study aimed i) to determine the total antioxidant capacities of Na2S and GYY4137, which are fast- and slow-releasing H2S donors, respectively, and ii) to test whether H2S donors are able to protect spermatozoa against oxidative stress. We found that Na2S and GYY4137 show different antioxidant properties, with the total antioxidant capacity of Na2S being mostly unstable and even undetectable at 150 µM. Moreover, both H2S donors preserve sperm motility and reduce acrosome loss, although the effects were both dose and donor dependent. Within the range of concentrations tested (3-300 µM), GYY4137 showed positive effects on sperm motility, whereas Na2S was beneficial at the lowest concentration but detrimental at the highest. Our findings show that Na2S and GYY4137 have different antioxidant properties and suggest that both H2S donors might be used as in vitro therapeutic agents against oxidative stress in sperm cells, although the optimal therapeutic range differs between the compounds.

• MeSH
• antioxidancia farmakologie   MeSH
• lidé MeSH
• morfoliny farmakologie   MeSH
• motilita spermií účinky léků   MeSH
• organothiofosforové sloučeniny farmakologie   MeSH
• oxidační stres účinky léků   MeSH
• prasata MeSH
• spermie účinky léků   MeSH
• sulfan metabolismus   MeSH
• sulfidy farmakologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antioxidancia MeSH
• GYY 4137 MeSH Prohlížeč
• morfoliny MeSH
• organothiofosforové sloučeniny MeSH
• sodium sulfide MeSH Prohlížeč
• sulfan MeSH
• sulfidy MeSH