Autism spectrum disorder (ASD) has been associated with disruptions in tryptophan (TRP) metabolism, affecting the production of key neuroactive metabolites. Investigating these metabolic pathways could yield valuable biomarkers for ASD severity and progression. We included 44 children with ASD and 44 healthy children, members of the same family. The average age in the ASD group was 10.7 years, while the average age in the control group was 9.4 years. Urinary tryptophan metabolites were quantified via liquid chromatography-mass spectrometry operating multiple reaction monitoring (MRM). Urinary creatinine was analyzed on an Advia 2400 analyzer using the Jaffe reaction. Statistical comparisons were made between ASD subgroups based on CARS scores. Our findings indicate that children with ASD have higher TRP concentrations (19.94 vs. 16.91; p = 0.04) than their siblings. Kynurenine (KYN) was found at higher levels in children with ASD compared to children in the control group (82.34 vs. 71.20; p = 0.86), although this difference was not statistically significant. The ASD group showed trends of higher KYN/TRP ratios and altered TRP/ indole-3-acetic acid (IAA) and TRP/5-hydroxyindoleacetic acid (5-HIAA) ratios, correlating with symptom severity. Although the numbers of the two groups were different, our findings suggest that mild and severe illnesses involve separate mechanisms. However, further comprehensive studies are needed to validate these ratios as diagnostic tools for ASD.

• Klíčová slova
• CARS, autism spectrum disorder, kynurenine, tryptophan,
• MeSH
• biologické markery * moč   MeSH
• dítě MeSH
• kynurenin * moč   metabolismus   MeSH
• kyselina hydroxyindoloctová moč   MeSH
• kyseliny indoloctové moč   metabolismus   MeSH
• lidé MeSH
• metabolom MeSH
• metabolomika * metody   MeSH
• mladiství MeSH
• poruchy autistického spektra * moč   metabolismus   MeSH
• studie případů a kontrol MeSH
• tryptofan * moč   metabolismus   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• biologické markery * MeSH
• indoleacetic acid MeSH Prohlížeč
• kynurenin * MeSH
• kyselina hydroxyindoloctová MeSH
• kyseliny indoloctové MeSH
• tryptofan * MeSH