Radiolabelled puromycin analogues will allow the quantification of protein synthesis through nuclear medicine-based imaging. A particularly useful application could be the non-invasive longitudinal visualisation of mycobacterial activity through direct quantification of puromycin binding. This study assesses the value of [68Ga]Ga-DOTA-puromycin in the visualisation of mycobacteria through positron emission tomography combined with magnetic resonance imaging (µPET/MRI). The radiopharmaceutical was produced by previously published and validated methods. [68Ga]Ga-DOTA-Puromycin imaging was performed on severe immunodeficient mice infected with Bacille Calmette-Guérin-derived M. Bovis (BCG). Acute and chronic infection stages were examined by µPET/MRI. A follow-up group of animals acted as controls (animals bearing S. aureus-derived infection and sterile inflammation) to assess tracer selectivity. [68Ga]Ga-DOTA-puromycin-µPET/MRI images revealed the acute, widespread infection within the right upper shoulder and armpit. Also, [68Ga]Ga-DOTA-puromycin signal sensitivity measured after a 12-week period was lower than that of [18F]FDG-PET in the same animals. A suitable correlation between normalised uptake values (NUV) and gold standard histopathological analysis confirms accurate tracer accumulation in viable bacteria. The radiopharmaceutical showed infection selectivity over inflammation but accumulated in both M. Bovis and S. Aureus, lacking pathogen specificity. Overall, [68Ga]Ga-DOTA-puromycin exhibits potential as a tool for non-invasive protein synthesis visualization, albeit without pathogen selectivity.

• Klíčová slova
• Fluorodeoxyglucose, Gallium-68, Molecular imaging, Mycobacterium tuberculosis, Positron emission tomography/magnetic resonance imaging,
• MeSH
• heterocyklické sloučeniny monocyklické chemie   MeSH
• magnetická rezonanční tomografie * metody   MeSH
• Mycobacterium bovis * MeSH
• mykobakteriózy diagnostické zobrazování   mikrobiologie   MeSH
• myši SCID MeSH
• myši MeSH
• organokovové sloučeniny MeSH
• pozitronová emisní tomografie * metody   MeSH
• radiofarmaka * chemie   MeSH
• radioizotopy galia * MeSH
• tuberkulóza diagnostické zobrazování   mikrobiologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• heterocyklické sloučeniny monocyklické MeSH
• organokovové sloučeniny MeSH
• radiofarmaka * MeSH
• radioizotopy galia * MeSH