Visualisation of in vivo protein synthesis during mycobacterial infection through [68Ga]Ga-DOTA-puromycin µPET/MRI
Jazyk angličtina Země Anglie, Velká Británie Médium electronic
Typ dokumentu časopisecké články
PubMed
39164329
PubMed Central
PMC11335739
DOI
10.1038/s41598-024-70200-4
PII: 10.1038/s41598-024-70200-4
Knihovny.cz E-zdroje
- Klíčová slova
- Fluorodeoxyglucose, Gallium-68, Molecular imaging, Mycobacterium tuberculosis, Positron emission tomography/magnetic resonance imaging,
- MeSH
- heterocyklické sloučeniny monocyklické chemie MeSH
- magnetická rezonanční tomografie * metody MeSH
- Mycobacterium bovis * MeSH
- mykobakteriózy diagnostické zobrazování mikrobiologie MeSH
- myši SCID MeSH
- myši MeSH
- organokovové sloučeniny MeSH
- pozitronová emisní tomografie * metody MeSH
- radiofarmaka * chemie MeSH
- radioizotopy galia * MeSH
- tuberkulóza diagnostické zobrazování mikrobiologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- heterocyklické sloučeniny monocyklické MeSH
- organokovové sloučeniny MeSH
- radiofarmaka * MeSH
- radioizotopy galia * MeSH
Radiolabelled puromycin analogues will allow the quantification of protein synthesis through nuclear medicine-based imaging. A particularly useful application could be the non-invasive longitudinal visualisation of mycobacterial activity through direct quantification of puromycin binding. This study assesses the value of [68Ga]Ga-DOTA-puromycin in the visualisation of mycobacteria through positron emission tomography combined with magnetic resonance imaging (µPET/MRI). The radiopharmaceutical was produced by previously published and validated methods. [68Ga]Ga-DOTA-Puromycin imaging was performed on severe immunodeficient mice infected with Bacille Calmette-Guérin-derived M. Bovis (BCG). Acute and chronic infection stages were examined by µPET/MRI. A follow-up group of animals acted as controls (animals bearing S. aureus-derived infection and sterile inflammation) to assess tracer selectivity. [68Ga]Ga-DOTA-puromycin-µPET/MRI images revealed the acute, widespread infection within the right upper shoulder and armpit. Also, [68Ga]Ga-DOTA-puromycin signal sensitivity measured after a 12-week period was lower than that of [18F]FDG-PET in the same animals. A suitable correlation between normalised uptake values (NUV) and gold standard histopathological analysis confirms accurate tracer accumulation in viable bacteria. The radiopharmaceutical showed infection selectivity over inflammation but accumulated in both M. Bovis and S. Aureus, lacking pathogen specificity. Overall, [68Ga]Ga-DOTA-puromycin exhibits potential as a tool for non-invasive protein synthesis visualization, albeit without pathogen selectivity.
Clinical for Nuclear Medicine University Hospital RWTH Aachen 52074 Aachen Germany
Department of Nuclear Medicine University of Pretoria Pretoria 0001 South Africa
Department of Radiopharmacy Charles University Prague 11000 Prague Czech Republic
Nuclear Medicine Research Infrastructure NPC Pretoria 0001 South Africa
Zobrazit více v PubMed
Treglia, G. Diagnostic performance of PubMed DOI PMC
Xu, T. & Chen, Y. Research progress of [ PubMed DOI
Signore, A., Glaudemans, A. W. J. M., Galli, F. & Rouzet, F. Imaging infection and inflammation. PubMed DOI PMC
Sathekge, M., Maes, A. & Van de Wiele, C. FDG-PET imaging in HIV infection and tuberculosis. PubMed DOI
Subramanian, S. PubMed DOI PMC
Yarmolinsky, M. B. & De la Haba, G. B. Inhibition by puromycin of amino acid incorporation into protein. PubMed DOI PMC
Nathans, D. Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains. PubMed DOI PMC
Miyamoto-Sato, E., Nemoto, N., Kobayashi, K. & Yanagawa, H. Spesific bonding of puromycin to full-length protein at the C-terminus. PubMed DOI PMC
Starck, S. R. & Roberts, R. W. Puromycin oligonucleotides reveal steric restrictions for ribosome entry and multiple modes of translation inhibition. PubMed DOI PMC
Septhaon, S. M. & Aibgirhio, F. I. Radiosynthesis of carbon-11 labeled puromycin as a potential PET candidate for imaging protein synthesis in vivo. PubMed DOI PMC
Velikyan, I. Prospective of PubMed DOI PMC
Lambrecht, F. Y. Evaluation of 99mTc-labeled antibiotics for infection detection. PubMed DOI
Eigner, S., Vera, D. B., Lebeda, O. & Henke, K. E.
Aviner, R. The science of puromycin: From studies of ribosome function to applications in biotechnology. PubMed DOI PMC
Liu, J., Yangquing, X., Stoleru, D. & Salic, A. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. PubMed DOI PMC
Liao, S., Eickelberg, O., Schmidt, E. P. & Yang, Y. Direct intrabronchial administration to improve the selective agent deposition within the mouse lung. PubMed DOI
Vorster, M., Maes, A., Van de Wiele, C. & Sathekge, M. PubMed DOI
Mackie and Mc Cartney Practical Medical Microbiology. Editors: J.G. Colle, A.G. Fraser, B.P. Marmion, A. Simmous, 4th ed, Publisher Churchill Living Stone, New York, Melborne, Sans Franscisco 1996.
